{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:29:07Z","timestamp":1787023747534,"version":"build-2736575974"},"reference-count":0,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[1991,7,1]],"date-time":"1991-07-01T00:00:00Z","timestamp":678326400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[1991,7]]},"abstract":"\n P\n olypeptide growth factors are a class<\/jats:sc>\n of potent natural biologic mediators which regulate many of the activities of wound healing including cell proliferation, migration, and metabolism. Platelet\u2010derived growth factor (PDGF) and insulin\u2010like growth factor\u2010I (IGF\u2010I) have been shown to regulate DNA and protein synthesis in bone cells in vitro and to interact synergistically to enhance soft tissue wound healing in vivo. We have hypothesized that the combination of PDGF and IGF\u2010I may, therefore, enhance regeneration of both the soft and hard tissue components of the periodontium. To test this hypothesis we performed conventional periodontal surgery on all 4 quadrants of the mouth of 13 beagle dogs with naturally occurring periodontal disease. Following flap reflection, degranulation, and root planing, all premolar teeth in 2 quadrants of each dog received a combination of 3\u03bcg of recombinant PDGF\u2010B and IGF\u2010I in a methylcellulose gel, while the premolar teeth in the contralateral quadrants received the gel alone. Teeth in 4 additional animals also received\n 125<\/jats:sup>\n I\u2010PDGF or\n 125<\/jats:sup>\n I\u2010IGF\u2010I in the treated sites. The clearance rate of the\n 125<\/jats:sup>\n I\u2010labeled protein, changes in local bone metabolism, and amount of new bone and cementum with inserting collagen fibers were measured. The clearance studies revealed that the half\u2010life of the factors at the site of application was 3.0 hours for IGF\u2010I and to 4.2 hours for PDGF\u2010B. Greater than 96% of the radio\u2010labeled proteins was cleared by 96 hours and no radioactivity was detected 2 weeks after application. There was a significant (\n P<\/jats:italic>\n < 0.01) 2\u2010fold increase in uptake of the bone\u2010seeking radiopharmaceutical Technetium 99\u2010MDP at 2 and 4 weeks in growth factor treated sites compared to controls, indicating that there was increased metabolic activity within the bone at these sites. Computer\u2010aided histologic analyses of biopsies obtained at 2 and 5 weeks post\u2010operatively revealed a significant (\n P<\/jats:italic>\n < 0.01), 5 to 10 fold increase in new bone and cementum in PDGF\u2010B\/IGF\u2010I treated sites at both time points compared to controls receiving the placebo gel. The height and total area of new bone continued to increase from 2 to 5 weeks. The new bone underwent a normal maturation process as judged by histologic appearance. A physiologic periodontal ligament space was also formed between the new bone and new cementum. There was no increase in ankylosis in the treated sites. This study demonstrates that the short term application of the combination of PDGF\u2010B and IGF\u2010I can significantly enhance the formation of the periodontal attachment apparatus during the early phases of wound healing following surgery.\n J Periodontol 1991; 62:458\u2013467<\/jats:italic>\n .\n <\/jats:p>","DOI":"10.1902\/jop.1991.62.7.458","type":"journal-article","created":{"date-parts":[[2010,10,28]],"date-time":"2010-10-28T14:04:20Z","timestamp":1288274660000},"page":"458-467","source":"Crossref","is-referenced-by-count":309,"title":["The Effects of Short\u2010Term Application of a Combination of Platelet\u2010Derived and Insulin\u2010Like Growth Factors on Periodontal Wound Healing"],"prefix":"10.1002","volume":"62","author":[{"given":"Samuel E.","family":"Lynch","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo Ruiz de","family":"Castilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ray C.","family":"Williams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher P.","family":"Kiritsy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. Howard","family":"Howell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael S.","family":"Reddy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harry N.","family":"Antoniades","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1991,7]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.1991.62.7.458","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T09:23:54Z","timestamp":1760865834000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.1991.62.7.458"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,7]]},"references-count":0,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1991,7]]}},"alternative-id":["10.1902\/jop.1991.62.7.458"],"URL":"https:\/\/doi.org\/10.1902\/jop.1991.62.7.458","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,7]]}}}