{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T11:02:06Z","timestamp":1762340526068,"version":"build-2065373602"},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1992,2,1]],"date-time":"1992-02-01T00:00:00Z","timestamp":696902400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[1992,2]]},"abstract":"<jats:p>T<jats:sc>he purpose of this study<\/jats:sc> was to use monoclonal antibodies to enumerate spirochetes in dental plaque, including the newly recognized pathogen\u2010related oral spirochete (PROS) and specific serovars of <jats:italic>Treponema denticola<\/jats:italic>. Plaque was collected from control subjects with no apparent periodontal disease and from sites of moderate to severe chronic periodontitis in patients with inflammatory periodontal disease. Individual monoclonal antibodies were used to determine whether spirochetes were present and then a doublestaining protocol was employed to count total spirochetes and specific treponemes in individual microscopic fields. Results indicate that spirochetes are more common at diseased sites and in subgingival plaque than at healthy sites or in supragingival plaque. Together PROS and <jats:italic>T. denticola<\/jats:italic> comprised the majority of all spirochetes in all samples and PROS and <jats:italic>T. denticola<\/jats:italic> serovars \u201cB\u201d and D were most numerous in plaque from patients with periodontitis. PROS were the majority of all spirochetes in supragingival plaque (76.2% \u00b1 23.8%) and subgingival plaque (60.9% \u00b1 19.1%) from periodontitis patients, significantly larger than the percentage of T. denticola serovar \u201cB\u201d (<jats:italic>P<\/jats:italic> &lt;.001 for both supragingival and subgingival plaque) and serovar D (<jats:italic>P<\/jats:italic> &lt;.01 for supragingival and <jats:italic>P<\/jats:italic> &lt;.001 for subgingival plaque). These observations indicate that PROS are the predominant spirochete in plaque from sites of patients with periodontitis, but other analytical approaches are necessary to determine if PROS or <jats:italic>T. denticola<\/jats:italic> are pathogenic. <jats:italic>J Periodontol 1992; 63:131\u2013136<\/jats:italic>.<\/jats:p>","DOI":"10.1902\/jop.1992.63.2.131","type":"journal-article","created":{"date-parts":[[2012,3,21]],"date-time":"2012-03-21T02:30:44Z","timestamp":1332297044000},"page":"131-136","source":"Crossref","is-referenced-by-count":45,"title":["Relative Proportions of Pathogen\u2010Related Oral Spirochetes (PROS) and <i>Treponema denticola<\/i> in Supragingival and Subgingival Plaque From Patients With Periodontitis"],"prefix":"10.1002","volume":"63","author":[{"given":"George R.","family":"Riviere","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathryn S.","family":"Elliot","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald F.","family":"Adams","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lloyd G.","family":"Simonson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorraine B.","family":"Forgas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela M.","family":"Nilius","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheila A.","family":"Lukehart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1992,2]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.1992.63.2.131","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T13:46:43Z","timestamp":1760881603000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.1992.63.2.131"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,2]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1992,2]]}},"alternative-id":["10.1902\/jop.1992.63.2.131"],"URL":"https:\/\/doi.org\/10.1902\/jop.1992.63.2.131","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"type":"print","value":"0022-3492"},{"type":"electronic","value":"1943-3670"}],"subject":[],"published":{"date-parts":[[1992,2]]}}}