{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T09:04:25Z","timestamp":1774515865793,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[1993,12,1]],"date-time":"1993-12-01T00:00:00Z","timestamp":754704000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[1993,12]]},"abstract":"<jats:p>B<jats:sc>ecause of their antimicrobial and anti\u2010inflammatory<\/jats:sc> properties, tetracyclines have been used systemically and locally in the treatment of periodontal disease. This study was done to evaluate the influence of doxycycline (De), a tetracycline, on fibroblast adherence to a protein coated surface and its cytotoxicity. Periodontal ligament derived fibroblasts (PLDFs) were either: 1) preincubated with Dc (0 to 100 \u03bcg\/ml) and then allowed to adhere to a gelatin\u2010coated surface or 2) adherence was first established and then Dc added to the system. After an appropriate time the number of PLDFs adherent, released, or lysed was estimated by a lactic dehydrogenase (LDH) assay. Preincubation of PLDFs in 25 \u03bcg Dc\/ml or higher concentrations significantly (<jats:italic>P<\/jats:italic>&lt;0.01) reduced the number of adherent cells. Fifty \u03bcg Dc\/ml and higher doses significantly (<jats:italic>P<\/jats:italic>&lt;0.01) increased PLDFs cytotoxicity as measured by LDH release. The same trend was noted if PLDFs were allowed to adhere and then subjected to the drug. Microscopic observation of fluorescein diacetate\/propidium iodide\u2010stained cells showed that attached\u2010spread cells pulled in, rounded up, and detached in proportion to the increased dose of Dc and the percentage of red\u2010stained cytotoxic cells rose in a similar manner. The data suggested that Dc can be cytotoxic and may inhibit PLDFs adherence and spread along a substratum. <jats:italic>J Periodontol 1993;64:1219\u20131224<\/jats:italic>.<\/jats:p>","DOI":"10.1902\/jop.1993.64.12.1219","type":"journal-article","created":{"date-parts":[[2012,3,21]],"date-time":"2012-03-21T20:27:50Z","timestamp":1332361670000},"page":"1219-1224","source":"Crossref","is-referenced-by-count":7,"title":["The Influence of Doxycycline on the Attachment of Fibroblasts to Gelatin\u2010Coated Surfaces and Its Cytotoxicity"],"prefix":"10.1002","volume":"64","author":[{"given":"Nobukazu","family":"Tsukuda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walter L.","family":"Gabler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1993,12]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.1993.64.12.1219","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T10:50:27Z","timestamp":1760871027000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.1993.64.12.1219"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,12]]},"references-count":0,"journal-issue":{"issue":"12","published-print":{"date-parts":[[1993,12]]}},"alternative-id":["10.1902\/jop.1993.64.12.1219"],"URL":"https:\/\/doi.org\/10.1902\/jop.1993.64.12.1219","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,12]]}}}