{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T06:53:14Z","timestamp":1770706394385,"version":"3.49.0"},"reference-count":0,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[1994,10,1]],"date-time":"1994-10-01T00:00:00Z","timestamp":780969600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[1994,10]]},"abstract":"<jats:p>W<jats:sc>e analyzed the cell\u2010cell adherence related<\/jats:sc> to CD11\/CD18 and CD18 mRNA in individuals with decreased CD11\/CD18 expression on their neutrophil surface. Epstein Barr virus\u2010transformed B cell lines were developed from one localized juvenile periodontitis (LJP) patient with decreased CD11\/CD18 in the peripheral blood neutrophils and without systemic diseases; two siblings with generalized prepubertal periodontitis (GPP) caused by leukocyte adhesion deficiency (LAD); another LJP patient; one localized prepubertal periodontitis. (LPP) patient; and two healthy subjects. Adhesion of leukocytes to each other was measured as cluster formation by aggregation assay. The length and the amount of CD18 mRNA expressed in the cell lines were analyzed by Northern blotting using the <jats:sup>32<\/jats:sup>P\u2010labeled CD18 cDNA. The coding region of the mRNA was analyzed by the reverse transcription\u2010polymerase chain reaction method. Base\u2010mismatches between CD18 mRNA and the <jats:sup>32<\/jats:sup>P\u2010labeled RNA probe synthesized from CD18 cDNA were analyzed by RNase protection assay. In the adherence assay, cells from the LJP patients with decreased CD11\/CD18 formed more clusters of smaller size and fewer cells than those of the other subjects. The cells from GPP and LAD patients did not aggregate and did not form clusters either in the absence or presence of PMA. There were no differences in the length and the amount of mRNA between the LJP patients and the other subjects, while GPP\u2010LAD patients expressed a small amount of long mRNA. The whole coding region (2,313 base pairs) of all subjects was amplified except for the GPPLAD patients, and the 5\u2032\u2010region (1,119 base pairs) was amplified from all subjects. Basemismatches were detected on the coding region from 965 to 1,450 nucleotides of CD18 mRNA in GPP\u2010LAD patients. No mismatch was detected on other regions of CD18 mRNA in any subject. These results suggest that the LJP patient with an anomaly of qualitative aggregation related to CD11\/CD18 is not related to sequence abnormalities of CD18 mRNA suggesting other mechanisms. In contrast, the adherence defect in two GPP\u2010LAD patients was related to heterogeneous molecular mutations on CD18. <jats:italic>J Periodontol 1994;65:949\u2013957<\/jats:italic>.<\/jats:p>","DOI":"10.1902\/jop.1994.65.10.949","type":"journal-article","created":{"date-parts":[[2012,3,20]],"date-time":"2012-03-20T23:12:55Z","timestamp":1332285175000},"page":"949-957","source":"Crossref","is-referenced-by-count":8,"title":["Molecular Basis of Leukocyte Adhesion Molecules in Early\u2010Onset Periodontitis Patients With Decreased CD11\/CD18 Expression on Leukocytes"],"prefix":"10.1002","volume":"65","author":[{"given":"Kyoko","family":"Katsuragi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shogo","family":"Takashiba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidemi","family":"Kurihara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoji","family":"Murayama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1994,10]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.1994.65.10.949","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.1994.65.10.949","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T11:20:19Z","timestamp":1760872819000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.1994.65.10.949"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,10]]},"references-count":0,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1994,10]]}},"alternative-id":["10.1902\/jop.1994.65.10.949"],"URL":"https:\/\/doi.org\/10.1902\/jop.1994.65.10.949","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,10]]}}}