{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T20:42:26Z","timestamp":1783629746201,"version":"3.55.0"},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1994,2,1]],"date-time":"1994-02-01T00:00:00Z","timestamp":760060800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[1994,2]]},"abstract":"<jats:p>T<jats:sc>he secretion of prostaglandin<\/jats:sc> E<jats:sub>2<\/jats:sub> (PGE<jats:sub>2<\/jats:sub>), tumor necrosis factor alpha (TNF\u03b1), interleukin 1 beta (IL\u20101\u03b2), and interleukin 6 (IL\u20106) by adherent mononuclear cells (AMNC) from 28 patients with early\u2010onset periodontitis was studied. The early onset\u2010periodontitis patients consisted of 12 patients with localized juvenile periodontitis (LJP) and 16 patients with severe generalized periodontitis (SGP). The AMNC responses to different concentrations of lipopolysaccharide (LPS) (<jats:italic>E. coli<\/jats:italic>) were determined in these 28 patients and compared to 14 healthy controls. Mediator levels in the supernatant were measured using radioimmunoassays for PGE<jats:sub>2<\/jats:sub>, IL\u20101\u03b2, and IL\u20106 determination and an enzyme linked immunosorbent assay for TNF\u03b1 levels. The mean age of the patients was 19.9 years for the LJP group, 30.4 years for SGP, and 28.0 years for the controls. The mean number of teeth per patient with attachment loss of &gt;6 mm was 4.75 in the LJP patients and 17.3 in the SGP group. In the absence of LPS, LJP AMNC secreted significantly more PGE<jats:sub>2<\/jats:sub> than unstimulated control or SGP AMNC, while similar baseline amounts of IL\u20101\u03b2, IL\u20106, and TNF\u03b1 were secreted by AMNC from the 3 patient groups. LPS stimulation resulted in the dose\u2010dependent secretion of significantly higher levels of PGE<jats:sub>2<\/jats:sub> by LJP AMNC compared to SGP AMNC which in turn secreted significantly more than controls. TNF\u03b1 secretion by LJP monocytes was significantly greater than the SGP and the control groups while IL\u20101\u03b2 secretion by the SGP AMNC was depressed compared to the other two patient groups. No significant difference in IL\u20106 secretion was noted among the 3 patient groups. These data suggest that the AMNC from the peripheral blood from each of the 3 patient groups differ considerably in their capacity to secrete inflammatory mediators. It is not clear as to whether these intergroup differences in systemic AMNC responsiveness represent an intrinsic or acquired difference in LPS responsiveness. <jats:italic>J Periodontol 1994;65:139\u2013146<\/jats:italic>.<\/jats:p>","DOI":"10.1902\/jop.1994.65.2.139","type":"journal-article","created":{"date-parts":[[2012,3,20]],"date-time":"2012-03-20T23:19:44Z","timestamp":1332285584000},"page":"139-146","source":"Crossref","is-referenced-by-count":105,"title":["The Secretion of PGE<sub>2<\/sub>, IL\u20101\u03b2, IL\u20106, and TNF\u03b1 by Adherent Mononuclear Cells From Early Onset Periodontitis Patients"],"prefix":"10.1002","volume":"65","author":[{"given":"Lior","family":"Shapira","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. Aubrey","family":"Soskolne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.N.","family":"Sela","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Offenbacher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Barak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1994,2]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.1994.65.2.139","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T10:37:42Z","timestamp":1760870262000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.1994.65.2.139"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,2]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1994,2]]}},"alternative-id":["10.1902\/jop.1994.65.2.139"],"URL":"https:\/\/doi.org\/10.1902\/jop.1994.65.2.139","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,2]]}}}