{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T11:01:58Z","timestamp":1762340518769,"version":"build-2065373602"},"reference-count":0,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[1994,7,1]],"date-time":"1994-07-01T00:00:00Z","timestamp":773020800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[1994,7]]},"abstract":"<jats:p>T<jats:sc>he presence and localization<\/jats:sc> of PMN\/neutrophil elastase and its endogenous inhibitor \u03b11\u2010proteinase inhibitor (\u03b11PI) were studied immunohistochemically in gingival tissue specimens collected from 9 adult periodontitis (AP) patients during flap surgery after the initial phase of periodontal therapy, and from 6 healthy controls with clinicallyhealthy periodontium upon surgical extraction of impacted third molars. In order to evaluate how periodontal tissue destructive events are reflected in gingival crevicular fluid (GCF), GCF samples were collected from the AP patients before any periodontal treatment and prior to flap surgery, from 5 localized juvenile periodontitis (LJP) patients, and from the controls. Elastase activity in the GCF was measured with the SAAVNA\u2010assay and the molecular forms and amount of \u03b11PI by Western\u2010 and dot\u2010blotting. Immunohistochemical staining for PMN elastase was strongly positive in the connective tissue, but not in the epithelium, of the AP patients' gingival tissue specimens. In the healthy gingival tissue specimens only a few elastase\u2010positive cells were present. Both in AP and in control gingival specimens, \u03b11PI was detected in the connective tissue and in the keratinized layer of the epithelium, however, its amount was markedly lower in the control specimens. Elevated levels of \u03b11PI and PMN elastase were detected in the GCF of all periodontitis patients when compared to controls. Based on the present findings we conclude that: 1) PMN elastase activity is associated with periodontal inflammation <jats:italic>in situ<\/jats:italic>, and increased activities are detected in AP GCF, but not in LJP GCF, and 2) \u03b11PI is present both in healthy and in inflamed periodontal tissues, but during periodontal inflammation its amount is increased, probably due to a compensatory extracellular matrix (ECM) \u2010protective mechanisms of the host's defense system. <jats:italic>J Periodontol 1994;65:702\u2013709<\/jats:italic>.<\/jats:p>","DOI":"10.1902\/jop.1994.65.7.702","type":"journal-article","created":{"date-parts":[[2012,3,21]],"date-time":"2012-03-21T01:10:44Z","timestamp":1332292244000},"page":"702-709","source":"Crossref","is-referenced-by-count":20,"title":["Elastase and \u03b1\u2010<sub>1<\/sub>\u2010Proteinase Inhibitor in Gingival Crevicular Fluid and Gingival Tissue in Adult and Juvenile Periodontitis"],"prefix":"10.1002","volume":"65","author":[{"given":"Tuula","family":"Ingman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timo","family":"Sorsa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petri","family":"Kangaspunta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yrj\u00f6 T.","family":"Konttinen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1994,7]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.1994.65.7.702","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.1994.65.7.702","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T10:59:41Z","timestamp":1760871581000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.1994.65.7.702"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,7]]},"references-count":0,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1994,7]]}},"alternative-id":["10.1902\/jop.1994.65.7.702"],"URL":"https:\/\/doi.org\/10.1902\/jop.1994.65.7.702","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"type":"print","value":"0022-3492"},{"type":"electronic","value":"1943-3670"}],"subject":[],"published":{"date-parts":[[1994,7]]}}}