{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:39:35Z","timestamp":1760920775613,"version":"build-2065373602"},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[1996,3,1]],"date-time":"1996-03-01T00:00:00Z","timestamp":825638400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[1996,3]]},"abstract":"<jats:p>P<jats:sc>revious studies from our laboratory<\/jats:sc> have demonstrated that freshly isolated peripheral blood monocytes from localized juvenile periodontitis (LJP) patients secrete more Prostaglandin E<jats:sub>2<\/jats:sub> (PGE<jats:sub>2<\/jats:sub>) after stimulation by lipopolysaccharide (LPS) than do monocytes from healthy subjects. However, it is not clear if the altered function of LJP monocytes is intrinsic to the cells or is induced by the persistent infection of the periodontium. The present study was designed to compare PGE<jats:sub>2<\/jats:sub> secretion by freshlyisolated peripheral blood monocytes (FIM) from LJP and control subjects to in vitro monocyte\u2010derived macrophages (MDM) from the same subjects. We also examined monocyte maturation into macrophage\u2010like cells and the cell\u2010surface expression of the LPS receptor, CD 14 during the culture period. FIM from LJP patients and controls were stimulated by different concentrations of LPS (0 to 30 \u03bcg\/ml) for 24 hours. These experiments were performed immediately after cell separation and after 10 days in culture, which allowed differentiation of monocytes into MDM. PGE<jats:sub>2<\/jats:sub> levels in the culture media were determined using a radioimmunoassay. Cell surface expression of CD71, a cell maturation marker, and CD 14 were assayed by cell\u2010ELISA in relation to \u03b2\u20102\u2010microglobulin. LPS\u2010stimulated FIM from LJP patients secreted 3 to 4 times more PGE<jats:sub>2<\/jats:sub> than control FIM at all LPS concentrations tested. After 10 days in culture, the LJP MDM secretion of PGE<jats:sub>2<\/jats:sub> reduced to control MDM level of PGE<jats:sub>2<\/jats:sub> secretion. These levels of PGE<jats:sub>2<\/jats:sub> secretion were comparable to PGE<jats:sub>2<\/jats:sub> secretion from FIM of controls. Cell maturation, as verified by CD71 expression, was found not to differ between the groups. However, the expression of CD 14 by LJP FIM was much lower than on control FIM (&amp;ap;50%). After 5 or 10 days in culture, MDM from both control and LJP subjects expressed comparable amounts of CD 14. The results suggest that in vitro conditions reverse the hypersensitivity of LJP monocytes to LPS into control levels and CD 14 expression is not correlated to the hyper\u2010responsiveness of the cells to LPS. <jats:italic>J Periodontol 1996;67:224\u2013228<\/jats:italic>.<\/jats:p>","DOI":"10.1902\/jop.1996.67.3.224","type":"journal-article","created":{"date-parts":[[2012,3,20]],"date-time":"2012-03-20T20:10:32Z","timestamp":1332274232000},"page":"224-228","source":"Crossref","is-referenced-by-count":21,"title":["Prostaglandin E<sub>2<\/sub> Secretion, Cell Maturation, and CD14 Expression by Monocyte\u2010Derived Macrophages From Localized Juvenile Periodontitis Patients"],"prefix":"10.1002","volume":"67","author":[{"given":"Lior","family":"Shapira","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. Aubrey","family":"Soskolne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas E.","family":"Van Dyke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1996,3]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.1996.67.3.224","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T13:16:38Z","timestamp":1760879798000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.1996.67.3.224"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,3]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1996,3]]}},"alternative-id":["10.1902\/jop.1996.67.3.224"],"URL":"https:\/\/doi.org\/10.1902\/jop.1996.67.3.224","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"type":"print","value":"0022-3492"},{"type":"electronic","value":"1943-3670"}],"subject":[],"published":{"date-parts":[[1996,3]]}}}