{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T21:56:44Z","timestamp":1761947804902,"version":"build-2065373602"},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[1996,3,1]],"date-time":"1996-03-01T00:00:00Z","timestamp":825638400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[1996,3]]},"abstract":"<jats:p>I<jats:sc>t has been proposed that healthy<\/jats:sc> gingiva is in a continuous state of wound repair. Thus, one might expect to find cells in normal gingiva producing growth factors associated with wound healing such as platelet\u2010derived growth factor B chain (PDGFB). One might also expect to find increased numbers of these cells or increased amounts of these growth factors in conditions which involve increased tissue volume such as drug\u2010induced gingival overgrowth (DGO). The purpose of this study was to quantify PDGF\u2010B gene expression and identify cells producing PDGF\u2010B in normal gingiva and DGO. Cyclosporine A (CSA) DGO was selected as a prototype of the overgrowth condition. Twelve patients with clinical CSA DGO and 12 patients with no DGO or history of drugs known to cause DGO were selected for study. Frozen sections of gingival specimens from these patients were subjected to in situ hybridization for PDGF\u2010B mRNA. Positive cells were counted and expressed as mean \u00b1 SEM cells\/mm<jats:sup>2<\/jats:sup> of lamina propria. Morphometric analysis revealed 6.2 \u00b1 1.9 cells\/mm<jats:sup>2<\/jats:sup> for control gingiva and 10.3 \u00b1 3.4 cells\/mm<jats:sup>2<\/jats:sup> for CSA DGO samples. There was no statistically significant difference between groups. PDGF\u2010B gene expression was measured in these cells and expressed as mean \u00b1 SEM silver grains\/cell. There was a significant upregulation of PDGF\u2010B gene expression in cells from the CSA DGO group (39.5 \u00b1 14.7 silver grains\/cell for normal gingiva vs. 255.3 \u00b1 77.1 silver grains\/cell for CSA DGO samples; <jats:italic>P<\/jats:italic> &lt; 0.001). The presence of PDGF\u2010B in these cells was confirmed in all cases by immunocytochemical localization. Additionally, PDGF\u2010B producing cells were identified as macrophages in sections taken from an additional patient with CSA DGO by double immunofluorescence labeling of the CD51 membrane marker for macrophages and intracellular PDGF\u2010B. These findings are consistent with the concept that healthy gingiva is in a continuous state of wound repair and support the hypothesis that CSA DGO is associated with enhanced macrophage PDGFB gene expression rather than an increase in the number of PDGF\u2010B producing macrophages. <jats:italic>J Periodontol 1996;67:264\u2013270<\/jats:italic>.<\/jats:p>","DOI":"10.1902\/jop.1996.67.3.264","type":"journal-article","created":{"date-parts":[[2012,3,20]],"date-time":"2012-03-20T20:10:32Z","timestamp":1332274232000},"page":"264-270","source":"Crossref","is-referenced-by-count":30,"title":["PDGF\u2010B Producing Cells and PDGF\u2010B Gene Expression in Normal Gingiva and Cyclosporine A\u2010Induced Gingival Overgrowth"],"prefix":"10.1002","volume":"67","author":[{"given":"Jacqueline M.","family":"Plemons","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Russell E.","family":"Dill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terry D.","family":"Rees","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara J.","family":"Dyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"May C.","family":"Ng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony M.","family":"Iacopino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1996,3]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.1996.67.3.264","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T13:16:36Z","timestamp":1760879796000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.1996.67.3.264"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,3]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1996,3]]}},"alternative-id":["10.1902\/jop.1996.67.3.264"],"URL":"https:\/\/doi.org\/10.1902\/jop.1996.67.3.264","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"type":"print","value":"0022-3492"},{"type":"electronic","value":"1943-3670"}],"subject":[],"published":{"date-parts":[[1996,3]]}}}