{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T11:02:06Z","timestamp":1762340526315,"version":"build-2065373602"},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[1997,3,1]],"date-time":"1997-03-01T00:00:00Z","timestamp":857174400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[1997,3]]},"abstract":"<jats:p>G<jats:sc>ingival crevicular fluid<\/jats:sc> (GGF) levels of the polymorphonuclear leukocyte (PMN) lysosomal enzyme beta\u2010glucuronidase (\u03b2G), the pro\u2010inflammatory cytokine interleukin 1\u03b2 (IL\u20101\u03b2), and Immunoglobulins (IgA, IgG, and IgM) were examined in 16 HIV seropositive (HIV+) and 10 HIV seronegative (HIV\u2013) injecting drug users (IDU). Each subject received a periodontal examination including assessment of probing depth, attachment level, bleeding on probing, and plaque and calculus accumulation. GCF was collected from the mesial surfaces of premolar and molar teeth using filter paper strips. Although HIV+ subjects had a significantly lower number of peripheral blood CD4+ T cells\/mm<jats:sup>3<\/jats:sup> compared to HIV\u2013subjects, there were no significant differences in mean probing depth, percentage of sites exhibiting bleeding on probing, or plaque and calculus accumulation between HIV\u2212 and HIV+ subjects. When the GCF components were analyzed, we found no significant differences between HIV\u2212 and HIV+ subjects in GCF levels of \u03b2G, IL\u20101\u03b2, IgA or IgM, but GCF levels of IgG were significantly increased in HIV+ subjects. When sites were categorized by probing depth, no differences in the levels of \u03b2G, IgA, IgG, and IgM existed between sites with probing depth s 3 mm compared to sites with probing depth \u2265 4 mm in both HIV\u2212 and HIV+ IDU. However, levels of IL\u20101\u03b2 in GCF were increased in the deeper sites (\u2265 4 mm) in HIV+ IDU when compared to sites with PD \u2264 3 mm. Analyzing GCF constituents in relation to the CD4 cell number, no differences were found between subjects with \u2264 400 or &gt; 400 CD4 cells\/mm3 with respect to the levels of IL\u20101\u03b2, IgG, and IgM. However, the level \u03b2G was significantly decreased in the HIV + IDU with \u2264 400 CD4 cells when compared to those with &gt; 400 CD4 cells\/mm<jats:sup>3<\/jats:sup>, while levels of IgA were significantly higher in HIV+ subjects with \u2264 400 CD4 cells\/mm<jats:sup>3<\/jats:sup>. Our results suggest that levels of IgG, and in immunodeficient subjects IgA were increased in GCF of HIV+ IDU while decreased levels of \u03b2G were found in immunodeficient HIV+ IDU. These findings may be local manifestions of systemic alterations and suggest that analysis of GCF may provide insight into the immune and inflammatory responses of HIV\u2010infected individuals to periodontal microorganisms. <jats:italic>J Periodontol 1997;68:249\u2013255<\/jats:italic>.<\/jats:p>","DOI":"10.1902\/jop.1997.68.3.249","type":"journal-article","created":{"date-parts":[[2012,3,20]],"date-time":"2012-03-20T18:05:24Z","timestamp":1332266724000},"page":"249-255","source":"Crossref","is-referenced-by-count":16,"title":["Inflammatory and Immune Mediators in Crevicular Fluid From HIV\u2010Infected Injecting Drug Users"],"prefix":"10.1002","volume":"68","author":[{"given":"John T.","family":"Grbic","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ira B.","family":"Lamster","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dennis","family":"Mitchell\u2010Lewis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1997,3]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.1997.68.3.249","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T10:23:20Z","timestamp":1760869400000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.1997.68.3.249"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,3]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1997,3]]}},"alternative-id":["10.1902\/jop.1997.68.3.249"],"URL":"https:\/\/doi.org\/10.1902\/jop.1997.68.3.249","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"type":"print","value":"0022-3492"},{"type":"electronic","value":"1943-3670"}],"subject":[],"published":{"date-parts":[[1997,3]]}}}