{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T20:13:56Z","timestamp":1792613636268,"version":"4.5.0"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[1998,1]]},"abstract":"<jats:p>T<jats:sc>he purpose of this study<\/jats:sc> was to investigate the distribution of several periodontopathic bacteria in adult periodontitis, their in vitro susceptibility to minocycline\u2010HCl, and whether the efficacy of the drug changes with a decrease in bacterial susceptibility. Twenty\u2010one patients (43 to 75 years old) with 62 periodontal lesions from pockets \u22654 mm participated in the study. After subgingival sampling, an ointment containing 2% minocycline\u2010HCl was applied locally to the selected pockets once a week for 4 weeks. The lesions were clinically examined after 1 and 4 weeks of administration. The distribution of the subgingival microorganisms included <jats:italic>Capnocytophaga sputigena<\/jats:italic> (37.1%), <jats:italic>Prevotella intermedia<\/jats:italic> (22.6%), <jats:italic>Porphyromonas gingivalis<\/jats:italic> (22.6%), Fuso<jats:italic>bacterium nucleatum<\/jats:italic> (20.1%), <jats:italic>Actinobacillus actinomycetemcomitans<\/jats:italic> (9.7%), and <jats:italic>Eikenella corrodens<\/jats:italic> (4.8%). The distribution was complex, with 76.8% of the sites containing 1 to 3 bacterial spieces. The minimum inhibitory concentration (MIC) of minocycline\u2010HCl for each organism showed that most were inhibited by a minocycline\u2010HCl concentration equal to or less than the MIC for reference strains. However, some clinical strains of <jats:italic>Prevotella intermedia<\/jats:italic> seemed to exihibit low susceptibility to minocycline\u2010HCl. There were no significant differences among sites with strains exhibiting low or normal susceptibility to minocycline\u2010HCl. The concentration of the drug applied to deep periodontal pockets inhibited the growth of most of the microorganisms investigated in this study. <jats:italic>J Periodontol 1998;69:92\u201399<\/jats:italic>.<\/jats:p>","DOI":"10.1902\/jop.1998.69.1.92","type":"journal-article","created":{"date-parts":[[2012,3,20]],"date-time":"2012-03-20T12:47:59Z","timestamp":1332247679000},"page":"92-99","source":"Crossref","is-referenced-by-count":10,"title":["Subgingival Distribution of Periodontopathic Bacteria in Adult Periodontitis and Their Susceptibility to Minocycline\u2010HCl"],"prefix":"10.1002","volume":"69","author":[{"given":"S.","family":"Hagiwara","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Takamatsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Tominaga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Umeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1998,1]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.1998.69.1.92","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T12:13:37Z","timestamp":1760876017000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.1998.69.1.92"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1998,1]]}},"alternative-id":["10.1902\/jop.1998.69.1.92"],"URL":"https:\/\/doi.org\/10.1902\/jop.1998.69.1.92","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,1]]}}}