{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:38:31Z","timestamp":1760920711233,"version":"build-2065373602"},"reference-count":0,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[1999,10,1]],"date-time":"1999-10-01T00:00:00Z","timestamp":938736000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[1999,10]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Semisynthetic tetracyclines used in the adjunctive treatment of inflammatory periodontal disease enhance collagen expression in induced periodontal lesions of rats. Polypeptide growth factors regulate key cellular events in tissue repair. The physiologically active androgen 5\u03b1\u2010dihydrotestosterone (DHT) stimulates bone and connective tissue turnover. It was relevant to study the effects of transforming growth factor beta (TGF\u2010\u03b2)\/platelet\u2010derived growth factor (PDGF) and minocycline alone and in combination on the formation of biologically effective androgens which can influence repair.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Confluent monolayer cultures of human gingival fibroblasts of the fifth through the ninth passage were incubated in Eagle's minimum essential medium, with 14C\u2010testosterone\/14C\u20104\u2010androstenedione in the presence or absence of optimal concentrations of TGF\u2010\u03b2\/PDGF\/minocycline (M), alone and in combination. At the end of a 24\u2010hour incubation period, the medium was analyzed for steroid metabolites and quantified using a radioisotope scanner.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> The androgen substrates 14C\u2010testosterone (14C\u2010T) and 14C\u20104\u2010androstenedione (14C\u20104\u2010A) were metabolized to DHT and 4\u2010androstenedione\/testosterone respectively. There were significant increases in the formation of DHT from 14C\u2010T in response to M, TGF\u2010\u03b2, and PDGF, alone and in combination (13 to 48%), compared with controls (n = 4; <jats:italic>P<\/jats:italic> &lt;0.01). The yields of 4\u2010androstenedione were also greater in response to these agents (31%; 3\u2010fold). When 14C\u20104\u2010A was used as substrate, there were 21 to 80% increases in the formation of DHT in response to these agents alone and in combination (n = 4; <jats:italic>P<\/jats:italic> &lt;0.01).<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> The biologically effective androgen metabolites formed in response to minocycline, TGF\u2010\u03b2, and PDGF can contribute to reparatory events in the inflamed periodontium. Judicious, adjunctive usage of the chemically\u2010modified tetracyclines in the treatment of periodontal diseases can obviate the risk of microbial resistance, with potential applications of their anti\u2010inflammatory and proanabolic effects in regenerative technology. <jats:italic>J Periodontol 1999;70:1136\u20101143.<\/jats:italic><\/jats:p>","DOI":"10.1902\/jop.1999.70.10.1136","type":"journal-article","created":{"date-parts":[[2005,2,2]],"date-time":"2005-02-02T06:00:28Z","timestamp":1107324028000},"page":"1136-1143","source":"Crossref","is-referenced-by-count":8,"title":["Implications of Minocycline, Platelet\u2010Derived Growth Factor, and Transforming Growth Factor\u2010Beta on Inflammatory Repair Potential in the Periodontium"],"prefix":"10.1002","volume":"70","author":[{"given":"M.","family":"Soory","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Virdi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1999,10]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.1999.70.10.1136","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T12:57:25Z","timestamp":1760878645000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.1999.70.10.1136"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,10]]},"references-count":0,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1999,10]]}},"alternative-id":["10.1902\/jop.1999.70.10.1136"],"URL":"https:\/\/doi.org\/10.1902\/jop.1999.70.10.1136","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"type":"print","value":"0022-3492"},{"type":"electronic","value":"1943-3670"}],"subject":[],"published":{"date-parts":[[1999,10]]}}}