{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:41:57Z","timestamp":1760920917307,"version":"build-2065373602"},"reference-count":0,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[1999,4,1]],"date-time":"1999-04-01T00:00:00Z","timestamp":922924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[1999,4]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Urokinase\u2010type plasminogen activator receptor (uPAR) presenting on the cell surface with glycosylphosphatidylinositol (GPI) anchor is a key component in the plasminogen activator (PA)\u2010plasmin system and plays a critical role in extracellular matrix degradation. It is believed that uPAR serves to localize and accelerate this generation system. In this study, we examined the levels of both uPA and uPAR in human gingival fibroblasts treated with <jats:italic>Porphyromonas gingivalis<\/jats:italic> lipopolysaccharide (LPS).<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Human gingival fibroblasts from the seventh to tenth doubling passages were plated at 5 x 10<jats:sub>4<\/jats:sub> cells per well in 24\u2010well plates. The confluent\u2010stage cells were cultured for 24 hours in \u03b1\u2010MEM medium containing 2% fetal calf serum, after which they were incubated with <jats:italic>P. gingivalis<\/jats:italic> LPS. PA activity was measured using plasminogen and plasmin substrate S2251.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> PA activity in the cell lysate was increased by LPS and reached maximum at 1 \u03bcg\/ml LPS after being incubated for 8 hours. PA activity released by phosphatidylinositol\u2010specific phospholipase C, which detaches the GPI anchor, was also increased by LPS. The activity was inhibited by amiloride, which is a specific inhibitor for uPA. LPS increased the protein and mRNA levels of both uPA and uPAR in gingival fibroblasts.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> These findings suggest that increase of the uPAR level by LPS may play an important role in the progression of periodontal diseases through pericellular proteolysis. <jats:italic>J Periodontol 1999;70:402\u2010408.<\/jats:italic><\/jats:p>","DOI":"10.1902\/jop.1999.70.4.402","type":"journal-article","created":{"date-parts":[[2005,2,2]],"date-time":"2005-02-02T06:06:53Z","timestamp":1107324413000},"page":"402-408","source":"Crossref","is-referenced-by-count":15,"title":["Increase of Urokinase\u2010Type Plasminogen Activator Receptor Expression in Human Gingival Fibroblasts by <i>Porphyromonas gingivalis<\/i> Lipopolysaccharide"],"prefix":"10.1002","volume":"70","author":[{"given":"Naomi","family":"Ogura","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideaki","family":"Nagura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshimitsu","family":"Abiko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1999,4]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.1999.70.4.402","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T13:39:17Z","timestamp":1760881157000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.1999.70.4.402"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,4]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1999,4]]}},"alternative-id":["10.1902\/jop.1999.70.4.402"],"URL":"https:\/\/doi.org\/10.1902\/jop.1999.70.4.402","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"type":"print","value":"0022-3492"},{"type":"electronic","value":"1943-3670"}],"subject":[],"published":{"date-parts":[[1999,4]]}}}