{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:19:07Z","timestamp":1760919547615,"version":"build-2065373602"},"reference-count":0,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[1999,5,1]],"date-time":"1999-05-01T00:00:00Z","timestamp":925516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[1999,5]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> The Gram\u2010negative facultatively anaerobic coccobacillus <jats:italic>Actinobacillus actinomycetemcomitans<\/jats:italic> is the major pathogen in localized juvenile periodontitis (LJP) and some forms of adult periodontitis (AP). <jats:italic>A. actinomycetemcomitans<\/jats:italic> can be grouped into 5 serotypes (a through e) based on differences in the carbohydrate moiety of cell surface lipopolysaccharide. The <jats:italic>A. actinomycetemcomitans<\/jats:italic> population is genetically heterogeneous. Since the studies on <jats:italic>A. actinomycetemcomitans<\/jats:italic> colonization have mostly applied only culture techniques, the clonality of the follow\u2010up isolates has not been established. Thus, it is possible that, although <jats:italic>A. actinomycetemcomitans<\/jats:italic> could be repeatedly isolated from an individual, the intitial colonizing strain was replaced by another strain. The aim of the study was to determine whether oral <jats:italic>A. actinomycetemcomitans<\/jats:italic> strains change spontaneously over time or after periodontal treatment.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> A total of 922 <jats:italic>A. actinomycetemcomitans<\/jats:italic> isolates were recovered from 115 subjects. From each subject <jats:italic>A. actinomycetemcomitans<\/jats:italic> isolates were obtained from 2 to 9 follow\u2010up samples 0.5 to 11.5 years apart. After the first sampling occasion, 99 subjects were treated for either LJP or AP, whereas the 16 non\u2010periodontitis subjects received no treatment. All <jats:italic>A. actinomycetemcomitans<\/jats:italic> isolates were serotyped and 235 isolates from 52 subjects genotyped with AP\u2010PCR and\/or with ribotyping.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Isolates of only one serotype, or non\u2010serotypeable isolates alone, were repeatedly found in 104 subjects; serotype a occurred in 25%, b in 33%, c in 23%, d in 5%, e in 7%, and non\u2010serotypeable isolates in 8% of these subjects. Two serotypes (or serotypeable isolates together with non\u2010serotypeable isolates) occurred simultaneously in 9 subjects and in each of these subjects at least one of the serotypes was detected at each sampling occasion. In one subject the initial serotype reappeared although a different serotype was once seen alone, whereas in another subject the initial serotype could not be recovered later. Identical genotypes of <jats:italic>A. actinomycetemcomitans<\/jats:italic> were repeatedly detected in each of 52 subjects with follow\u2010up isolates of the same serotype.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> The results showed that spontaneous or treatmentinduced change in the oral <jats:italic>A. actinomycetemcomitans<\/jats:italic> strain(s) is extremely rare and that colonization with the same strain(s) seems to be remarkably persistent. <jats:italic>J Periodontol 1999;70:504\u2010509.<\/jats:italic><\/jats:p>","DOI":"10.1902\/jop.1999.70.5.504","type":"journal-article","created":{"date-parts":[[2005,2,2]],"date-time":"2005-02-02T05:57:19Z","timestamp":1107323839000},"page":"504-509","source":"Crossref","is-referenced-by-count":37,"title":["Persistence of Oral Colonization by the Same <i>Actinobacillus actinomycetemcomitans<\/i> Strain(s)"],"prefix":"10.1002","volume":"70","author":[{"given":"Maria H.","family":"Saarela","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ba\u015fak","family":"Do\u011fan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satu","family":"Alaluusua","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sirkka","family":"Asikainen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1999,5]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.1999.70.5.504","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T08:47:35Z","timestamp":1760863655000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.1999.70.5.504"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,5]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1999,5]]}},"alternative-id":["10.1902\/jop.1999.70.5.504"],"URL":"https:\/\/doi.org\/10.1902\/jop.1999.70.5.504","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"type":"print","value":"0022-3492"},{"type":"electronic","value":"1943-3670"}],"subject":[],"published":{"date-parts":[[1999,5]]}}}