{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:21:06Z","timestamp":1760919666338,"version":"build-2065373602"},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2000,3,1]],"date-time":"2000-03-01T00:00:00Z","timestamp":951868800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2000,3]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> We have cloned the gene for a 40\u2010kDa outer membrane protein (40\u2010kDa OMP) from <jats:italic>Porphyromonas gingivalis<\/jats:italic> 381. The recombinant (r)40\u2010kDa OMP has become the subject of considerable interest because of its potential role in the development of a vaccine useful for passive immunization. To develop such a vaccine, it is essential to fully understand the functions of anti\u2010r40\u2010kDa OMP antibody in the host defense against <jats:italic>P. gingivalis.<\/jats:italic> To that end, we developed a panel of monoclonal antibodies by immunizing mice with purified r40\u2010kDa OMP. The objective of this study was to determine the bactericidal activity on <jats:italic>P. gingivalis<\/jats:italic> by the IgG1 monoclonal antibody Pg\u2010ompA2.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Bacterial growth measurement, a complementmediated anti\u2010P. gingivalis assay based on [<jats:sup>3<\/jats:sup>H]thymidine uptake, and a 14C\u2010release assay were performed to test the bactericidal activity of Pg\u2010ompA2 to <jats:italic>P. gingivalis.<\/jats:italic><\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> In the presence of complement, Pg\u2010ompA2 was lethal to P. gingivalis 381 as well as to the more virulent <jats:italic>P. gingivalis<\/jats:italic> strains, including ATCC 53977 and W83. Using component\u2010defi\u2010cient complement, we determined that Pg\u2010ompA2 killed <jats:italic>P. gingivalis<\/jats:italic> by activating both the classical and alternative complement pathways.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> Pg\u2010ompA2 has an in vitro complement\u2010mediated bactericidal activity to P. gingivalis. Pg\u2010ompA2 may contribute to the development of a local immunotherapy that can be applied in the gingival crevice of a patient with <jats:italic>P. gingivalis<\/jats:italic> related periodontitis, or be a vaccine candidate. <jats:italic>J Periodontol 2000;71:368\u2010375.<\/jats:italic><\/jats:p>","DOI":"10.1902\/jop.2000.71.3.368","type":"journal-article","created":{"date-parts":[[2005,2,2]],"date-time":"2005-02-02T06:28:11Z","timestamp":1107325691000},"page":"368-375","source":"Crossref","is-referenced-by-count":28,"title":["Bactericidal Activity of a Monoclonal Antibody Against a Recombinant 40\u2010kDa Outer Membrane Protein of <i>Porphyromonas gingivalis<\/i>"],"prefix":"10.1002","volume":"71","author":[{"given":"Mitsunobu","family":"Katoh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeno","family":"Saito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hisashi","family":"Takiguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshimitsu","family":"Abiko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2000,3]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2000.71.3.368","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T09:02:50Z","timestamp":1760864570000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2000.71.3.368"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,3]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2000,3]]}},"alternative-id":["10.1902\/jop.2000.71.3.368"],"URL":"https:\/\/doi.org\/10.1902\/jop.2000.71.3.368","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"type":"print","value":"0022-3492"},{"type":"electronic","value":"1943-3670"}],"subject":[],"published":{"date-parts":[[2000,3]]}}}