{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T01:38:02Z","timestamp":1772933882572,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2000,5,1]],"date-time":"2000-05-01T00:00:00Z","timestamp":957139200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2000,5]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Localized juvenile periodontitis (LJP) is an earlyonset periodontal disease associated with neutrophil dysfunction, including defective chemotaxis, reduced protein kinase C (PKC) activity, and reduced calcium entry. These observations are important because reduced availability of cytosolic\u2010free calcium concentration in the cell will have detrimental consequences for the numerous cytosolic calcium concentration\u2010dependent pathways. In particular, there is a direct relationship between Ca2+ flux and the cell activation enzyme PKC. In this report, we focused on the mechanism of calcium entry, investigating a newly described molecule, calcium influx factor (CIF). CIF is thought to be a second messenger for the opening of membrane calcium channels when intracellular calcium stores are depleted. We examined CIF activity in neutrophils from normal subjects and LJP patients.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Neutrophils from 11 LJP patients, 3 adult periodontitis (AP) patients, and 12 normal subjects were isolated from peripheral venous blood. CIF was extracted with thapsigargin, a Ca<jats:sup>2+<\/jats:sup>\u2010ATPase inhibitor, from isolated neutrophils and CIF activity measured using a <jats:sup>45<\/jats:sup>CaCl<jats:sub>2<\/jats:sub> uptake assay.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> The CIF activity in neutrophils from LJP patients ranged from 98.9 to 281.5 units\/mg protein (mean = 180.2 \u00b1 56.3) and from 291.9 to 755.5 units\/mg protein (mean = 528.8 \u00b1 153.8) in non\u2010periodontal disease controls. CIF activity in AP patients was also measured and found to be similar to controls. The CIF activity in LJP patients was statistically significantly reduced compared to that in normal subjects (<jats:italic>P<\/jats:italic> &lt;0.001).<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> This study suggests that CIF activity may be important determinant in neutrophil abnormalities in LJP. <jats:italic>J Periodontol 2000;71:797\u2010802.<\/jats:italic><\/jats:p>","DOI":"10.1902\/jop.2000.71.5.797","type":"journal-article","created":{"date-parts":[[2005,2,2]],"date-time":"2005-02-02T06:31:29Z","timestamp":1107325889000},"page":"797-802","source":"Crossref","is-referenced-by-count":16,"title":["Defective Calcium Influx Factor Activity in Neutrophils From Patients With Localized Juvenile Periodontitis"],"prefix":"10.1002","volume":"71","author":[{"given":"Kazuyuki","family":"Shibata","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martha L.","family":"Warbington","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara J.","family":"Gordon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidemi","family":"Kurihara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas E.","family":"Van Dyke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2000,5]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2000.71.5.797","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T11:36:38Z","timestamp":1760873798000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2000.71.5.797"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,5]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2000,5]]}},"alternative-id":["10.1902\/jop.2000.71.5.797"],"URL":"https:\/\/doi.org\/10.1902\/jop.2000.71.5.797","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,5]]}}}