{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:12:10Z","timestamp":1791353530259,"version":"4.3.1"},"reference-count":0,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2000,6,1]],"date-time":"2000-06-01T00:00:00Z","timestamp":959817600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2000,6]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> This study aimed to determine the relationships among interleukin (IL)\u20108 and granulocyte elastase levels in gingival crevicular fluid (GCF) and the concomitant presence of periodontopathogens in untreated adult periodontitis.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> GCF and subgingival plaque samples were collected from 16 patients with untreated adult periodontitis and 10 healthy control subjects. IL\u20108 levels were determined by enzymelinked immunosorbent assay (ELISA). Granulocyte elastase was analyzed with a neutrophilic granulocyte\u2010specific, low molecular weight and chromogenic substrate, L\u2010pyroglutamyl\u2010Lprolyl\u2010L\u2010valine\u2010p\u2010nitroanilide, and the maximal rate of elastase activity (MR\u2010EA) was calculated. Five DNA probes were used to detect the presence of <jats:italic>A. actinomycetemcomitans (A.a.),<\/jats:italic> <jats:italic>B. forsythus (B.f.), P. gingivalis (P.g.), P. intermedia (P.i.),<\/jats:italic> and <jats:italic>T. denticola (T.d.).<\/jats:italic><\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Lower IL\u20108 concentrations and higher granulocyte elastase activities were found in patients than in healthy controls as well as in diseased conditions co\u2010infected with <jats:italic>B.f., P.g., P.i.,<\/jats:italic> and <jats:italic>T.d.<\/jats:italic> as compared to healthy conditions without the target species (<jats:italic>P<\/jats:italic> &lt;0.05). IL\u20108 concentrations were positively correlated with MR\u2010EA levels in the periodontitis conditions coinfected with <jats:italic>B.f., P.g., P.i.,<\/jats:italic> and <jats:italic>T.d.<\/jats:italic> (<jats:italic>P<\/jats:italic> &lt;0.05). A wide range of IL\u20108 concentrations was found among 15 patients when the periodontitis condition was characterized by co\u2010infection with <jats:italic>B.f., P.g., P.i.,<\/jats:italic> and <jats:italic>T.d.<\/jats:italic> MR\u2010EA levels in the high IL\u20108 group of subjects were significantly higher than those in the low IL\u20108 group of subjects (<jats:italic>P<\/jats:italic> &lt;0.01).<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> The present study shows that the local hostbacteria interactions in untreated periodontitis are diverse in terms of the intensity of inflammatory responses measured by IL\u20108\u2013related granulocyte elastase activity in GCF. This might reflect different phases of the inflammatory response due to shifts in host\u2010bacteria interactions and therefore be indicative of a range of periodontal disease activity levels. <jats:italic>J Periodontol 2000;71:929\u2010939.<\/jats:italic><\/jats:p>","DOI":"10.1902\/jop.2000.71.6.929","type":"journal-article","created":{"date-parts":[[2005,2,2]],"date-time":"2005-02-02T06:33:12Z","timestamp":1107325992000},"page":"929-939","source":"Crossref","is-referenced-by-count":61,"title":["Interleukin\u20108 and Granulocyte Elastase in Gingival Crevicular Fluid in Relation to Periodontopathogens in Untreated Adult Periodontitis"],"prefix":"10.1002","volume":"71","author":[{"given":"Lijian","family":"Jin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgitta","family":"S\u00f6der","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esmonde F.","family":"Corbet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2000,6]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2000.71.6.929","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T09:28:34Z","timestamp":1760866114000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2000.71.6.929"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,6]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2000,6]]}},"alternative-id":["10.1902\/jop.2000.71.6.929"],"URL":"https:\/\/doi.org\/10.1902\/jop.2000.71.6.929","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,6]]}}}