{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:39:07Z","timestamp":1761154747729,"version":"build-2065373602"},"reference-count":0,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2000,7,1]],"date-time":"2000-07-01T00:00:00Z","timestamp":962409600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2000,7]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> T cell cytokine profiles in the spleens and anti\u2010<jats:italic>Porphyromonas gingivalis<\/jats:italic> antibodies in the sera of <jats:italic>P. gingivalis<\/jats:italic>\u2010immunized BALB\/c (H\u20102<jats:sup>d<\/jats:sup>), CBA\/CaH (H\u20102<jats:sup>k<\/jats:sup>), C57BL6 (H\u20102<jats:sup>b<\/jats:sup>), and DBA\/2J (H\u20102<jats:sup>d<\/jats:sup>, C5 deficient) mice were examined.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Mice were immunized either by intraperitoneal injections of <jats:italic>P. gingivalis<\/jats:italic> outer membrane antigens and Freund's incomplete adjuvant weekly for 3 weeks or sham\u2010immunized with PBS and adjuvant, followed by subcutaneous challenge with live organisms 1 week after the final immunization. Spleens were excised and blood samples collected by heart puncture at 0 and 7 days after challenge. Splenic CD4 and CD8 cells were stained for intracytoplasmic interleukin (IL)\u20104, interferon (IF)\u2010gamma, and IL\u201010 and levels of anti\u2010<jats:italic>P. gingivalis<\/jats:italic> antibodies in the serum samples determined by ELISA.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Lesion sizes in immunized BALB\/c mice remained stable for the 7\u2010day experimental period. Immunized CBA\/CaH and C57BL6 mice exhibited large lesions at day 1 reducing by day 7 particularly in the latter strain. Lesions in immunized DBA\/2J mice were still larger than the other strains at day 7. With the exception of DBA\/2J mice, sham\u2010immunized mice demonstrated lesions which did not show signs of healing by day 7. T cell cytokine responses in sham\u2010immunized mice at day 0 were low, increasing to a variable degree by day 7 after challenge in the 4 strains. Immunized BALB\/c mice demonstrated intermediate T cell responses while generally exhibiting a stronger IFN\u2010gamma response than IL\u20104 or IL\u201010. Immunized CBA\/CaH and C57BL6 mice showed weak T cell cytokine responses while immunized DBA\/2J displayed the strongest T cell responses particularly in regard to IL\u20104 positive cells. Sham\u2010immunized mice had low levels of serum anti\u2010<jats:italic>P. gingivalis<\/jats:italic> antibody levels at day 0 with levels increasing significantly by day 7 after challenge. Antibody levels in immunized mice seemed to correlate with lesion sizes. Immunized C57BL6 mice had the highest antibody levels followed by CBA\/CaH, BALB\/c with DBA\/2J exhibiting low levels. The T cell and B cell antibody responses in each strain appeared to exhibit an inverse relationship.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> This study has shown that genetic differences at the level of H\u20102 haplotype induce variations in the local and T and B cell responses to <jats:italic>P. gingivalis<\/jats:italic> antigens. The responses of DBA\/2J mice which have the same haplotype as BALB\/c mice suggest that factors other than H\u20102 haplotype such as the C5 deficiency may influence this immune response. The significance of the specific antibody and T cell responses and of their inverse relationship to susceptibility to periodontal disease remains to be determined. <jats:italic>J Periodontol 2000;71:1130\u20101138.<\/jats:italic><\/jats:p>","DOI":"10.1902\/jop.2000.71.7.1130","type":"journal-article","created":{"date-parts":[[2005,2,2]],"date-time":"2005-02-02T06:36:05Z","timestamp":1107326165000},"page":"1130-1138","source":"Crossref","is-referenced-by-count":21,"title":["The Influence of Genetic Variation on the Splenic T Cell Cytokine and Specific Serum Antibody Responses to <i>Porphyromonas gingivalis<\/i> in Mice"],"prefix":"10.1002","volume":"71","author":[{"given":"Erica","family":"Gemmell","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tracey A.","family":"Winning","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorothy A.","family":"Grieco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip S.","family":"Bird","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory J.","family":"Seymour","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2000,7]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2000.71.7.1130","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T11:39:11Z","timestamp":1760873951000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2000.71.7.1130"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,7]]},"references-count":0,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2000,7]]}},"alternative-id":["10.1902\/jop.2000.71.7.1130"],"URL":"https:\/\/doi.org\/10.1902\/jop.2000.71.7.1130","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"type":"print","value":"0022-3492"},{"type":"electronic","value":"1943-3670"}],"subject":[],"published":{"date-parts":[[2000,7]]}}}