{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T17:06:59Z","timestamp":1764781619431,"version":"build-2065373602"},"reference-count":0,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2001,10,1]],"date-time":"2001-10-01T00:00:00Z","timestamp":1001894400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2001,10]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Serum concentrations of immunoglobulin G2 (IgG2) are elevated in localized aggressive periodontitis (LAgP) patients, and secretory products of monocytes from LAgP patients enhance IgG2 responses of lymphocytes from healthy subjects. Furthermore, genes regulating production of interleukin (IL)\u20101 influence the risk for both aggressive periodontitis (AgP) and chronic periodontitis. These observations, and the fact that IgG2 dominates responses to carbohydrates from <jats:italic>Actinobacillus actinomycetemcomitans<\/jats:italic> and <jats:italic>Porphyromonas gingivalis<\/jats:italic>, prompted the hypothesis that IL\u20101\u03b1, IL\u20101\u03b2, and IL\u2010RA may help regulate human IgG2 responses.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Human peripheral blood leukocytes (PBL) were stimulated in culture with pokeweed mitogen (PWM); the levels of available IL\u20101 gene products were manipulated; and the effect on IgG2 production was monitored. Manipulations of IL\u20101 were accomplished by adding specific neutralizing monoclonal antibodies or recombinant IL\u20101RA, IL\u20101\u03b1, or IL\u20101\u03b2.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Blocking the IL\u20101 receptor with IL\u20101RA or neutralizing IL\u20101\u03b1 or IL\u20101\u03b2 with specific antibody dramatically suppressed IgG2 production (50% to 70%). Additional IL\u20101\u03b1 did not compensate for neutralized IL\u20101\u03b2, and additional IL\u20101\u03b2 did not compensate for neutralized IL\u20101\u03b1, suggesting the 2 monokines have separate roles in promoting IgG2. Furthermore, combinations of anti\u2010IL\u20101\u03b1 and anti\u2010IL\u20101\u03b2 were more inhibitory than either antibody alone, and IL\u20101\u03b1 and IL\u20101\u03b2 in combination appeared to work additively in promoting IgG2. Moreover, PBL cultures from a group of LAgP patients with high IgG2 levels had elevated levels of IL\u20101\u03b2.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> IL\u20101\u03b1 and IL\u20101\u03b2 appear to have critical and nonredundant roles in the generation and regulation of potent IgG2 responses, which appear to be important in human responses to carbohydrate\u2010bearing bacteria. <jats:italic>J Periodontol 2001;72:1332\u20101339.<\/jats:italic><\/jats:p>","DOI":"10.1902\/jop.2001.72.10.1332","type":"journal-article","created":{"date-parts":[[2005,2,2]],"date-time":"2005-02-02T06:43:44Z","timestamp":1107326624000},"page":"1332-1339","source":"Crossref","is-referenced-by-count":15,"title":["Non\u2010Redundant Roles for Interleukin\u20101\u03b1 and Interleukin\u20101\u03b2 in Regulating Human IgG2"],"prefix":"10.1002","volume":"72","author":[{"given":"Yuichi","family":"Ishihara","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji\u2010Bo","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed","family":"Fakher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Al M.","family":"Best","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harvey A.","family":"Schenkein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzanne E.","family":"Barbour","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John G.","family":"Tew","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,10]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2001.72.10.1332","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T14:34:54Z","timestamp":1760884494000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2001.72.10.1332"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,10]]},"references-count":0,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2001,10]]}},"alternative-id":["10.1902\/jop.2001.72.10.1332"],"URL":"https:\/\/doi.org\/10.1902\/jop.2001.72.10.1332","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"type":"print","value":"0022-3492"},{"type":"electronic","value":"1943-3670"}],"subject":[],"published":{"date-parts":[[2001,10]]}}}