{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T15:48:33Z","timestamp":1790264913402,"version":"4.1.0"},"reference-count":0,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2001,10,1]],"date-time":"2001-10-01T00:00:00Z","timestamp":1001894400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2001,10]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> The aim of the present study was to identify <jats:italic>Treponema socranskii<\/jats:italic> in addition to <jats:italic>Treponema denticola<\/jats:italic> and <jats:italic>Porphyromonas gingivalis<\/jats:italic> by polymerase chain reaction (PCR), and to clarify the relationship between the presence of these microorganisms and the severity of clinical periodontal parameters.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Saliva and subgingival plaque collected from 123 subjects (38 aggressive periodontitis patients, 65 chronic periodontitis patients, 20 healthy patients) were subjected to PCR to detect the aforementioned 3 microorganisms.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Detection frequencies of <jats:italic>T. socranskii, T. denticola<\/jats:italic>, and <jats:italic>P. gingivalis<\/jats:italic> in plaque samples from aggressive periodontitis patients (71.1%, 73.7%, 84.2%, respectively) and chronic periodontitis patients (89.2%, 93.8%, 95.3%) were much higher than those from healthy subjects (30%, 5.0%, 10.0%). In aggressive and chronic periodontitis patients, these 3 species of bacteria were detected frequently at sites that showed deep periodontal pockets and severe attachment loss. The percentage of these bacteria\u2010positive sites increased as the gingival index score of chronic periodontitis patients increased. <jats:italic>T. socranskii<\/jats:italic> was frequently detected together with <jats:italic>T. denticola<\/jats:italic> or <jats:italic>P. gingivalis<\/jats:italic> at the same sites, and coexistence of these microorganisms was frequently observed in deep periodontal pockets of aggressive periodontitis patients.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> <jats:italic>T. socranskii, T. denticola<\/jats:italic>, and <jats:italic>P. gingivalis<\/jats:italic> were frequently detected in periodontitis patients by PCR. The prevalence of these 3 microorganisms was correlated with various clinical parameters. Taken together, our findings suggest that <jats:italic>T. socranskii, T. denticola<\/jats:italic>, and <jats:italic>P. gingivalis<\/jats:italic> are associated with the severity of periodontal tissue destruction. <jats:italic>J Periodontol 2001;72:1354\u20101363.<\/jats:italic><\/jats:p>","DOI":"10.1902\/jop.2001.72.10.1354","type":"journal-article","created":{"date-parts":[[2005,2,2]],"date-time":"2005-02-02T06:43:44Z","timestamp":1107326624000},"page":"1354-1363","source":"Crossref","is-referenced-by-count":120,"title":["<i>Treponema socranskii, Treponema denticola,<\/i> and <i>Porphyromonas gingivalis<\/i> Are Associated With Severity of Periodontal Tissue Destruction"],"prefix":"10.1002","volume":"72","author":[{"given":"Yasuo","family":"Takeuchi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Umeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuo","family":"Sakamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshimi","family":"Benno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isao","family":"Ishikawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,10]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2001.72.10.1354","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T14:34:55Z","timestamp":1760884495000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2001.72.10.1354"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,10]]},"references-count":0,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2001,10]]}},"alternative-id":["10.1902\/jop.2001.72.10.1354"],"URL":"https:\/\/doi.org\/10.1902\/jop.2001.72.10.1354","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,10]]}}}