{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:33:11Z","timestamp":1760920391614,"version":"build-2065373602"},"reference-count":0,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2001,12,1]],"date-time":"2001-12-01T00:00:00Z","timestamp":1007164800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2001,12]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Limited information is available as to whether local cellular immunity in early\u2010onset periodontitis (EOP) subjects harboring <jats:italic>Actinobacillus actinomycetemcomitans<\/jats:italic> (<jats:italic>Aa<\/jats:italic>) differs from that in patients without <jats:italic>Aa<\/jats:italic>. In addition, the effect of scaling and root planing on various lymphocyte subsets is described rather sparsely.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> In 10 subjects with early\u2010onset periodontitis harboring <jats:italic>Aa<\/jats:italic> (EOP\u2010<jats:italic>Aa<\/jats:italic>) and in 10 subjects without <jats:italic>Aa<\/jats:italic> (EOP\u2010non<jats:italic>Aa<\/jats:italic>), clinical measurements were recorded and gingival biopsies were performed before and after scaling and root planing. The specimens were cut into serial sections; using the alkaline phosphatase\u2010antialkaline phosphatase technique, monoclonal antibodies to CD20 (B cells), CD30 (plasma cells), and CD45RO (T\u2010memory cells) were applied as well as polyclonal antibodies to alpha, gamma, and mu chains (Ig A, G, and M). Cells were counted from an area of 0.25 mm<jats:sup>2<\/jats:sup> in areas showing the largest infiltration.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Before therapy, mean counts of all cell phenotypes were found to be markedly enhanced in the EOP\u2010<jats:italic>Aa<\/jats:italic> group compared to EOP\u2010non<jats:italic>Aa<\/jats:italic> subjects. Following scaling and root planing, the numbers of all phenotypes decreased in both groups. However, comparing the data before and after therapy in the EOP\u2010<jats:italic>Aa<\/jats:italic> group, the <jats:italic>P<\/jats:italic> value was &lt;0.05 only for CD30\u2010positive cells. In the EOP\u2010non<jats:italic>Aa<\/jats:italic> group, the differences before and after therapy reached statistical significance (<jats:italic>P<\/jats:italic> &lt;0.05) for all cell counts, except for IgMpositive cells. Furthermore, reduction of probing depth and gain of clinical attachment reached the 0.05 level of statistical significance only in EOP\u2010non<jats:italic>Aa<\/jats:italic> subjects.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> In EOP subjects harboring <jats:italic>Aa<\/jats:italic>, inflammatory cell subsets were detected in 2\u2010 to 3\u2010fold higher numbers compared to patients without <jats:italic>Aa<\/jats:italic>. Scaling and root planing resulted in a decrease of all cell phenotypes studied in individuals without <jats:italic>Aa<\/jats:italic>, whereas in subjects with <jats:italic>Aa<\/jats:italic>, the only significant decrease that was seen occurred in plasma cells. <jats:italic>J Periodontol 2001;72:1713\u20101719.<\/jats:italic><\/jats:p>","DOI":"10.1902\/jop.2001.72.12.1713","type":"journal-article","created":{"date-parts":[[2005,2,2]],"date-time":"2005-02-02T06:48:16Z","timestamp":1107326896000},"page":"1713-1719","source":"Crossref","is-referenced-by-count":7,"title":["Some Effects of Non\u2010Surgical Therapy on Gingival Inflammatory Cell Subsets in Patients With Early\u2010Onset Periodontitis Associated With <i>Actinobacillus actinomycetemcomitans<\/i>"],"prefix":"10.1002","volume":"72","author":[{"given":"J\u00f6rg W.","family":"Kleinfelder","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anton","family":"Sculean","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dieter E.","family":"Lange","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2001.72.12.1713","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T11:45:00Z","timestamp":1760874300000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2001.72.12.1713"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,12]]},"references-count":0,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2001,12]]}},"alternative-id":["10.1902\/jop.2001.72.12.1713"],"URL":"https:\/\/doi.org\/10.1902\/jop.2001.72.12.1713","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"type":"print","value":"0022-3492"},{"type":"electronic","value":"1943-3670"}],"subject":[],"published":{"date-parts":[[2001,12]]}}}