{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T00:16:32Z","timestamp":1790381792696,"version":"4.1.0"},"reference-count":0,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2001,8,1]],"date-time":"2001-08-01T00:00:00Z","timestamp":996624000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2001,8]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Localized aggressive periodontitis (LAgP) is associated with neutrophil dysfunction including defective chemotaxis and reduced calcium influx factor activity. Nitric oxide (NO) and its enzyme, nitric oxide synthase (NOS), have been suggested to be involved in chemotaxis. Some reports, however, were unable to detect either NO or NOS in human neutrophils. In this study, we focused on NOS activity in LAgP neutrophils and examined the involvement of NOS in chemotaxis of normal neutrophils and NOS activity in neutrophils from normal subjects and patients with LAgP.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Neutrophils from 10 normal subjects and 10 LAgP patients were isolated from peripheral venous blood. Membrane associated\u2010NOS (MA\u2010NOS) and soluble NOS (S\u2010NOS) were extracted from cells with or without FMLP stimulation. NOS activity was measured using the radiolabeled L\u2010arginine to L\u2010citrulline conversion assay.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> N<jats:sup>\u03c9<\/jats:sup>\u2010nitro\u2010L\u2010arginine methyl ester (L\u2010NAME), an inhibitor of NOS, significantly inhibited FMLP\u2010induced chemotaxis (<jats:italic>P<\/jats:italic> &lt;0.01) and dibutyryl cGMP, an activator of cGMP\u2010dependent protein kinase, significantly attenuated the inhibition by LNAME (<jats:italic>P<\/jats:italic> &lt;0.01). Unstimulated and FMLP\u2010stimulated MA\u2010NOS activity in LAgP neutrophils was statistically significantly higher than that in normal neutrophils (<jats:italic>P<\/jats:italic> &lt;0.05). S\u2010NOS activity in LAgP neutrophils was higher than that in normal neutrophils.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> This study suggests that NOS is present in human neutrophils and may be involved in FMLP\u2010induced chemotaxis in normal neutrophils. NOS activity is increased in LAgP and is negatively correlated to chemotaxis response. <jats:italic>J Periodontol 2001;72:1052\u20101058.<\/jats:italic><\/jats:p>","DOI":"10.1902\/jop.2001.72.8.1052","type":"journal-article","created":{"date-parts":[[2005,2,2]],"date-time":"2005-02-02T07:00:21Z","timestamp":1107327621000},"page":"1052-1058","source":"Crossref","is-referenced-by-count":28,"title":["Nitric Oxide Synthase Activity in Neutrophils From Patients With Localized Aggressive Periodontitis"],"prefix":"10.1002","volume":"72","author":[{"given":"Kazuyuki","family":"Shibata","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha L.","family":"Warbington","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara J.","family":"Gordon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidemi","family":"Kurihara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas E.","family":"Van Dyke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,8]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2001.72.8.1052","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T13:38:37Z","timestamp":1760881117000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2001.72.8.1052"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,8]]},"references-count":0,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2001,8]]}},"alternative-id":["10.1902\/jop.2001.72.8.1052"],"URL":"https:\/\/doi.org\/10.1902\/jop.2001.72.8.1052","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,8]]}}}