{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:30:57Z","timestamp":1760920257559,"version":"build-2065373602"},"reference-count":0,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2002,11,1]],"date-time":"2002-11-01T00:00:00Z","timestamp":1036108800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2002,11]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Cyclosporin A (CsA) is a potent immunosuppressant used to prevent organ transplant rejection and to treat various autoimmune diseases. CsA\u2010induced gingival overgrowth (CsA GO) is the most widely seen side effect of this drug; its pathogenesis is not completely understood. The aim of this study was to identify and compare matrix metalloproteinase\u20101 (MMP\u20101) and tissue inhibitor of metalloproteinase\u20101 (TIMP\u20101) levels in gingival fibroblast cultures of tissues derived from renal transplant patients receiving CsA and exhibiting gingival overgrowth and from periodontally healthy control subjects.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Gingival overgrowth samples were obtained from patients undergoing therapy with CsA, and control tissues were obtained from systemically healthy donors. Gingival fibroblasts were grown using explant cultures. Three different study groups were identified: 1) CsA GO fibroblast culture; 2) CsA\u2010treated healthy gingival fibroblast culture (H+CsA); and 3) healthy gingival fibroblast culture (H). The levels of MMP\u20101 and TIMP\u20101 in these groups of gingival fibroblasts were analyzed by enzymelinked immunoabsorbent assay (ELISA).<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> The levels of TIMP\u20101 were significantly lower in CsA GO than H (<jats:italic>P<\/jats:italic> &lt;0.05). There was no statistically significant difference in the levels of MMP\u20101 between H and CsA GO (<jats:italic>P<\/jats:italic> \u20100.505). The ratio of MMP\u20101 to TIMP\u20101 was significantly higher in CsA GO than H (<jats:italic>P<\/jats:italic> &lt;0.05).<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> The results of this study indicate that CsA therapy does not have a significant effect on MMP\u20101 levels. However, low TIMP\u20101 levels can be an important factor in the pathogenesis of CsA GO, since the balance between MMP\u20101 and TIMP\u20101 levels was changed by CsA. <jats:italic>J Periodontol 2002;73:1273\u20101278.<\/jats:italic><\/jats:p>","DOI":"10.1902\/jop.2002.73.11.1273","type":"journal-article","created":{"date-parts":[[2005,2,2]],"date-time":"2005-02-02T07:15:30Z","timestamp":1107328530000},"page":"1273-1278","source":"Crossref","is-referenced-by-count":12,"title":["Evaluation of Matrix Metalloproteinase\u20101 and Tissue Inhibitor of Metalloproteinase\u20101 Levels in Gingival Fibroblasts of Cyclosporin A\u2010Treated Patients"],"prefix":"10.1002","volume":"73","author":[{"given":"G\u00fclay","family":"T\u00fcter","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhittin A.","family":"Serdar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehmet","family":"Yalim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ismet S.","family":"G\u00fcrhan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K\u00f6ksal","family":"Balo\u015f","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,11]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2002.73.11.1273","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T11:07:26Z","timestamp":1760872046000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2002.73.11.1273"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,11]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2002,11]]}},"alternative-id":["10.1902\/jop.2002.73.11.1273"],"URL":"https:\/\/doi.org\/10.1902\/jop.2002.73.11.1273","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"type":"print","value":"0022-3492"},{"type":"electronic","value":"1943-3670"}],"subject":[],"published":{"date-parts":[[2002,11]]}}}