{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T17:52:49Z","timestamp":1778781169048,"version":"3.51.4"},"reference-count":0,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2002,12,1]],"date-time":"2002-12-01T00:00:00Z","timestamp":1038700800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Our previous reports found that basic fibroblast growth factor (FGF\u20102; bFGF) influences the proliferation and extracellular matrix production of periodontal ligament (PDL) cells. In this study, we examined FGF\u20102 expression in gingival epithelium and the effect of FGF\u20102 on proliferative responses by gingival epithelial (GE) cells.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Human GE cells were isolated from healthy gingival epithelium, and the mRNA expression of FGF\u20102 and FGF receptors (FGFRs) was examined by reverse transcription\u2010polymerase chain reaction (RT\u2010PCR). The distribution of FGF\u20102 in gingival tissues was detected by immunohistological analysis using the monoclonal antibody for human recombinant FGF\u20102, which was newly established and designated as BF\u20102. Further, the proliferative responses of GE cells to FGF\u20102 were investigated by measuring [<jats:sup>3<\/jats:sup>H]\u2010thymidine uptake.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> RT\u2010PCR revealed that GE cells express FGFR\u20101, FGFR\u20102, FGFR\u20103, and FGFR\u20104 mRNA; however, not that of FGF\u20102. Employing immunohistochemical staining with BF\u20102, FGF\u20102 was observed localized in the intercellular spaces of gingival epithelium, though not in the cytoplasm of epithelial cells. Interestingly, staining by BF\u20102 in the intercellular spaces was diminished after treatment of the tissue sections with heparitinase. Further, an in vitro analysis revealed that FGF\u20102 enhanced the proliferative responses of human GE cells. However, costimulation with fetal calf serum inhibited the FGF\u20102\u2010induced proliferation of GE cells, whereas the same costimulation synergistically enhanced FGF\u20102\u2010induced PDL cell proliferation.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> FGF\u20102 is anchored in the intercellular spaces of gingival epithelium via heparansulfate and may regulate the growth and cytodifferentiation of GE cells via cell\u2010type specific receptors. <jats:italic>J Periodontol 2002;73:1467\u20101473.<\/jats:italic><\/jats:p>","DOI":"10.1902\/jop.2002.73.12.1467","type":"journal-article","created":{"date-parts":[[2005,2,2]],"date-time":"2005-02-02T07:16:15Z","timestamp":1107328575000},"page":"1467-1473","source":"Crossref","is-referenced-by-count":46,"title":["Effects of Basic Fibroblast Growth Factor on Human Gingival Epithelial Cells"],"prefix":"10.1002","volume":"73","author":[{"given":"Shin\u2010ichi","family":"Takayama","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joji","family":"Yoshida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Hirano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Okada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinya","family":"Murakami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,12]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2002.73.12.1467","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T10:46:59Z","timestamp":1760870819000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2002.73.12.1467"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,12]]},"references-count":0,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2002,12]]}},"alternative-id":["10.1902\/jop.2002.73.12.1467"],"URL":"https:\/\/doi.org\/10.1902\/jop.2002.73.12.1467","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,12]]}}}