{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T20:37:07Z","timestamp":1792701427187,"version":"4.5.0"},"reference-count":0,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,4,1]],"date-time":"2002-04-01T00:00:00Z","timestamp":1017619200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2002,4]]},"abstract":"<jats:p>\n                    <jats:bold>Background:<\/jats:bold>\n                    Different periodontopathogenic microbiota have been associated with periodontal diseases in several populations. The present investigation determined the subgingival microbiota of untreated chronic periodontitis Brazilians using the checkerboard DNA\u2010DNA hybridization technique.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods:<\/jats:bold>\n                    Twenty\u2010five periodontitis patients (mean age, 41 \u00b1 2; mean probing depth [PD], 3.3 \u00b1 0.2; mean attachment level [AL], 3.6 \u00b1 0.2) with no history of previous periodontal therapy and a control group of 14 healthy subjects (mean age, 34 \u00b1 0.6; mean PD, 1.8 \u00b1 0.2; mean AL, 1.7 \u00b1 0.1) were selected. Measurements of PD, AL, bleeding on probing, plaque accumulation, and suppuration were recorded at 6 sites\/tooth. Subgingival plaque samples were obtained from 4 sites in each tooth\/subject in both groups. The presence and levels of 41 subgingival species were determined in 4,032 plaque samples using whole genomic DNA probes and the checkerboard method.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    Periodontal pathogens, as well as some unusual species (\n                    <jats:italic>E. faecalis, E. coli<\/jats:italic>\n                    and\n                    <jats:italic>Bartonella sp.<\/jats:italic>\n                    ), were detected significantly more often and\/or in higher levels in the periodontitis group (\n                    <jats:italic>P<\/jats:italic>\n                    &lt;0.05). Most species were more frequently detected in interproximal sites.\n                    <jats:italic>B. forsythus, P. gingivalis, E. nodatum<\/jats:italic>\n                    , and\n                    <jats:italic>F. nucleatum ss vincentii<\/jats:italic>\n                    showed a significant positive correlation with mean PD and AL (\n                    <jats:italic>P<\/jats:italic>\n                    &lt;0.05).\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions:<\/jats:bold>\n                    The subgingival microbiota of Brazilians with untreated chronic periodontitis were complex, including high proportions of periodontopathogens commonly found in other populations, as well as some unusual species.\n                    <jats:italic>J Periodontol 2002;73:360\u2010369.<\/jats:italic>\n                  <\/jats:p>","DOI":"10.1902\/jop.2002.73.4.360","type":"journal-article","created":{"date-parts":[[2005,2,2]],"date-time":"2005-02-02T07:22:14Z","timestamp":1107328934000},"page":"360-369","source":"Crossref","is-referenced-by-count":117,"title":["Subgingival Microbiota of Brazilian Subjects With Untreated Chronic Periodontitis"],"prefix":"10.1002","volume":"73","author":[{"given":"Ana Paula V.","family":"Colombo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo P.","family":"Teles","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Cyn\u00e9sia","family":"Torres","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata","family":"Souto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Wilson","family":"Rosal\u00e9m","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Cl\u00e1udia S.","family":"Mendes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milton","family":"Uzeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,4]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2002.73.4.360","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T10:41:26Z","timestamp":1760870486000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2002.73.4.360"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,4]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2002,4]]}},"alternative-id":["10.1902\/jop.2002.73.4.360"],"URL":"https:\/\/doi.org\/10.1902\/jop.2002.73.4.360","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,4]]}}}