{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T22:28:57Z","timestamp":1790720937107,"version":"4.1.0"},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,3,1]],"date-time":"2003-03-01T00:00:00Z","timestamp":1046476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2003,3]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Increased collagen and extracellular matrix deposition within the gingiva is the main characteristic feature of hereditary gingival fibromatosis (HGF). To date, it is not well established if these events are a consequence of alterations in the collagen and other extracellular matrix molecules synthesis or disturbances in the homeostatic equilibrium between synthesis and degradation of extracellular matrix molecules. Cytokines are important regulators of expression of the profibrogenic genes, including type I collagen and its molecular chaperone heat shock protein (Hsp)47 and proteolytic enzymes degrading extracellular matrix such as matrix metalloproteinases\u20101 and \u20102 (MMP\u20101 and MMP\u20102).<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> In this study, we analyzed the expression and production of type I collagen, Hsp47, MMP\u20101, and MMP\u20102 in normal gingiva (NG) and HGF fibroblasts, and investigated the effects of transforming growth factorbeta1 (TGF\u2010\u03b21), interleukin\u20106 (IL\u20106) and interferon\u2010gamma (IFN\u2010\u03b3) on the expression of these genes by NG and HGF fibroblasts.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Our results obtained from semi\u2010quantitative reverse transcription\u2010polymerase chain reactions (RTPCR), Western blots, enzyme\u2010linked immunosorbent assays (ELISA), and enzymographies clearly demonstrated that the expression and production of type I collagen and Hsp47 were significantly higher in fibroblasts from HGF than from NG, whereas MMP\u20101 and MMP\u20102 expression and production were lower in fibroblasts from HGF patients. Addition of TGF\u2010\u03b21 and IL\u20106, which are produced in greater amounts by HGF fibroblasts, promoted an increase in type I collagen and Hsp47 and a decrease in MMP\u20101 and MMP\u20102 expression. IFN\u2010\u03b3 reduced both type I collagen and Hsp47 expression, whereas it had a slight effect on the expression of MMP\u20101 and MMP\u20102.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> These patterns of expression and production suggest that enhanced TGF\u2010\u03b21 and IL\u20106 production simultaneously increase the synthesis and reduce the proteolytic activities of fibroblasts from patients with HGF, which may favor the accumulation of extracellular matrix observed in patients with this condition. <jats:italic>J Periodontol 2003;74:296\u2010306<\/jats:italic>.<\/jats:p>","DOI":"10.1902\/jop.2003.74.3.296","type":"journal-article","created":{"date-parts":[[2005,2,2]],"date-time":"2005-02-02T07:37:59Z","timestamp":1107329879000},"page":"296-306","source":"Crossref","is-referenced-by-count":68,"title":["Effect of Transforming Growth Factor\u2010\u03b21, Interleukin\u20106, and Interferon\u2010\u03b3 on the Expression of Type I Collagen, Heat Shock Protein 47, Matrix Metalloproteinase (MMP)\u20101 and MMP\u20102 by Fibroblasts from Normal Gingiva and Hereditary Gingival Fibromatosis"],"prefix":"10.1002","volume":"74","author":[{"given":"H.","family":"Martelli\u2010Junior","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Cotrim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Graner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.J.","family":"Sauk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.D.","family":"Coletta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,3]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2003.74.3.296","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T13:55:47Z","timestamp":1760882147000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2003.74.3.296"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,3]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2003,3]]}},"alternative-id":["10.1902\/jop.2003.74.3.296"],"URL":"https:\/\/doi.org\/10.1902\/jop.2003.74.3.296","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,3]]}}}