{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T21:32:12Z","timestamp":1791063132494,"version":"4.1.0"},"reference-count":0,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2003,4,1]],"date-time":"2003-04-01T00:00:00Z","timestamp":1049155200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2003,4]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Localized aggressive periodontitis (LAgP) is a disease characterized by rapid loss of alveolar bone in teeth of otherwise healthy patients. Neutrophils from LAgP patients have been shown to exhibit diminished chemotaxis and low levels of formyl peptide receptor (FPR) surface expression. A recent study has associated LAgP with 2 polymorphisms in the FPR: 110Phe\u2192Ser and 126Cys\u2192Trp.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> We transfected Chinese hamster ovary cells with wtFPR, FPR\u2010110Phe\u2192Ser, FPR\u2010126Cys\u2192Trp, or FPR\u2010110 Phe\u2192Ala and determined their surface expression of FPR, their ligand binding affinity, their G\u2010protein coupling, and their chemotaxis toward N\u2010formyl\u2010methionyl\u2010leucyl\u2010phenylalanine (FMLP).<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> FPR\u2010110Phe\u2192Ser mutants failed to show any significant surface expression or chemotaxis toward FMLP. FPR\u2010126Cys\u2192Trp mutants exhibited slightly lower than normal binding affinity, markedly lower G\u2010protein coupling response, and markedly lower chemotaxis toward FMLP than that observed with wtFPR. We also analyzed another FPR\u2010Phe110 mutant, FPR\u2010110Phe\u2192Ala, to ascertain what the effect of mutating this residue might be in a mutant that could be expressed on the cell surface. The FPR\u2010110Phe\u2192Ala mutant demonstrated markedly lower surface expression, normal ligand binding affinity, markedly lower G\u2010protein coupling, and markedly lower chemotaxis toward FMLP.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> Our data substantiate the hypothesis that the chemotactic defects observed in LAgP patients are due at least in part to molecular alterations in the FPR. The FPR\u2010110Phe\u2192Ser polymorphism appears to be more defective than the FPR\u2010126Cys\u2192Trp polymorphism, indicating that patients with the former polymorphism might be expected to exhibit a more severe form of aggressive periodontitis. <jats:italic>J Periodontol 2003; 74:475\u2010484<\/jats:italic><\/jats:p>","DOI":"10.1902\/jop.2003.74.4.475","type":"journal-article","created":{"date-parts":[[2005,2,2]],"date-time":"2005-02-02T07:38:16Z","timestamp":1107329896000},"page":"475-484","source":"Crossref","is-referenced-by-count":23,"title":["Mutations of F110 and C126 of the Formyl Peptide Receptor Interfere with G\u2010Protein Coupling and Chemotaxis"],"prefix":"10.1002","volume":"74","author":[{"given":"Barbara E.","family":"Jones","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heini M.","family":"Miettinen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Algirdas J.","family":"Jesaitis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John S.","family":"Mills","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,4]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2003.74.4.475","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T08:41:13Z","timestamp":1760863273000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2003.74.4.475"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,4]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2003,4]]}},"alternative-id":["10.1902\/jop.2003.74.4.475"],"URL":"https:\/\/doi.org\/10.1902\/jop.2003.74.4.475","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,4]]}}}