{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T20:39:51Z","timestamp":1772138391018,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2003,4,1]],"date-time":"2003-04-01T00:00:00Z","timestamp":1049155200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2003,4]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Matrix metalloproteinases (MMPs) have been shown to be involved in the degradation of the extracellular matrix (ECM). In particular, MMP\u20102 and MMP\u20109 (gelatinase A and gelatinase B, respectively) have been identified as the predominant MMPs during periodontitis. Recent studies have indicated that a novel transmembrane MMP, mebrane\u2010type 1 matrix metalloproteinase (MT1\u2010MMP), can activate pro\u2010MMP\u20102 in tumor metastasis. This study aims to elucidate the presence and localization of MT1\u2010MMP and MMP\u20102 in periodontitis in a rat model. Methods: In 2 groups of 40\u2010day\u2010old male Sprague\u2010Dawley rats, periodontitis was initiated by ligating floss around maxillary second molars. A group of control animals were left untreated. Maxillary dentoalveolar segments were isolated after 7 and 21 days postinduction and were prepared for gross and radiographic analysis of bone loss and for histological analysis. Samples were also prepared for gel zymography to detect the presence of MMP\u20102, and for Northern blot analysis and in situ hybridization with MT1\u2010MMP probes.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> MMP\u20102 expression increased at 21 days following ligature placement, in conjunction with MT1\u2010MMP expression. MT1\u2010MMP mRNA expression was observed in epithelial cells, fibroblasts, and in multinucleated cells in the periodontium.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> Our data suggest that MT1\u2010MMP may play a role in extracellular matrix degradation during periodontitis, in concert with MMP\u20102 and other proteinases. <jats:italic>J Periodontol 2003;74: 494\u2010500.<\/jats:italic><\/jats:p>","DOI":"10.1902\/jop.2003.74.4.494","type":"journal-article","created":{"date-parts":[[2005,2,2]],"date-time":"2005-02-02T07:38:16Z","timestamp":1107329896000},"page":"494-500","source":"Crossref","is-referenced-by-count":29,"title":["Membrane Type (MT)1\u2010Matrix Metalloproteinase (MMP) and MMP\u20102 Expression in Ligature\u2010Induced Periodontitis in the Rat"],"prefix":"10.1002","volume":"74","author":[{"given":"Ron","family":"Achong","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ichiro","family":"Nishimura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hema","family":"Ramachandran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas H.","family":"Howell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph P.","family":"Fiorellini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadeem Y.","family":"Karimbux","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,4]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2003.74.4.494","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T08:41:14Z","timestamp":1760863274000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2003.74.4.494"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,4]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2003,4]]}},"alternative-id":["10.1902\/jop.2003.74.4.494"],"URL":"https:\/\/doi.org\/10.1902\/jop.2003.74.4.494","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,4]]}}}