{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T23:07:05Z","timestamp":1777417625435,"version":"3.51.4"},"reference-count":0,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2003,7,1]],"date-time":"2003-07-01T00:00:00Z","timestamp":1057017600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> It has been reported that <jats:italic>Porphyromonas gingivalis<\/jats:italic> is closely associated with chronic periodontitis and its detection has been recommended as a routine marker for periodontal diagnosis. The purpose of this study was to evaluate the sensitivity and specificity of a DNA probe to detect <jats:italic>P. gingivalis<\/jats:italic> in adult Chinese periodontitis patients as well as to find a rapid and convenient method to detect <jats:italic>P. gingivalis<\/jats:italic> in clinical practice.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> A total of 26 bacterial strains (20 reference strains and 6 clinical isolates) were collected, of which 5 were <jats:italic>P. gingivalis<\/jats:italic> and 21 were heterologous species. A DNA fragment of 542 bp, which encodes the fimbriae subunit protein (fimA) of <jats:italic>P. gingivalis<\/jats:italic>, was obtained by polymerase chain reaction (PCR) and molecular cloning techniques and used to construct the DNA probe, labeled with <jats:sup>32<\/jats:sup>P or with digoxigenin. The constructed DNA probe was used to detect <jats:italic>P. gingivalis<\/jats:italic> in specimens collected from the periodontal pockets of 100 patients clinically confirmed with chronic periodontitis. One hundred periodontally healthy persons served as a control group.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Positive reactions were seen in all 5 strains of <jats:italic>P. gingivalis<\/jats:italic> while no visible reaction was found in other species. The DNA probe was capable of detecting as few as 100 <jats:italic>P. gingivalis<\/jats:italic> cells in samples. A significant difference in the positive rates of <jats:italic>P. gingivalis<\/jats:italic> between the periodontitis patients and the control group was found (<jats:italic>P<\/jats:italic> &lt;0.01). In addition, the amount of detected <jats:italic>P. gingivalis<\/jats:italic> was positively correlated with the extent of tooth mobility, depth of periodontal pockets, and patient age (<jats:italic>P<\/jats:italic> &lt;0.01).<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> The DNA probe is specific and sensitive for the detection of <jats:italic>P. gingivalis<\/jats:italic> in chronic periodontitis specimens and may be a suitable method for the clinical diagnosis of chronic periodontitis. <jats:italic>J Periodontol 2003;74:1000\u20101006<\/jats:italic>.<\/jats:p>","DOI":"10.1902\/jop.2003.74.7.1000","type":"journal-article","created":{"date-parts":[[2005,2,2]],"date-time":"2005-02-02T07:41:50Z","timestamp":1107330110000},"page":"1000-1006","source":"Crossref","is-referenced-by-count":6,"title":["Species\u2010Specific DNA Probe for the Detection of <i>Porphyromonas gingivalis<\/i> from Adult Chinese Periodontal Patients and Healthy Subjects"],"prefix":"10.1002","volume":"74","author":[{"family":"Luchuan\u2010Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Xiujie\u2010Wen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Huixia\u2010He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Junnan\u2010Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Changhua\u2010Ji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,7]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2003.74.7.1000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T09:46:39Z","timestamp":1760867199000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2003.74.7.1000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,7]]},"references-count":0,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2003,7]]}},"alternative-id":["10.1902\/jop.2003.74.7.1000"],"URL":"https:\/\/doi.org\/10.1902\/jop.2003.74.7.1000","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,7]]}}}