{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T12:02:05Z","timestamp":1789387325979,"version":"build-2803163510"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Low\u2010dose doxycycline (LDD) is recognized to have non\u2010antimicrobial properties that can therapeutically modulate the host response. The aim of the present randomized, double\u2010blind, placebo\u2010controlled, parallel\u2010arm study was to examine the effectiveness of LDD in combination with non\u2010surgical periodontal therapy, compared to non\u2010surgical periodontal therapy alone, on gingival crevicular fluid (GCF) matrix metalloproteinase\u20108 (MMP\u20108) levels and clinical parameters over a 12\u2010month period in patients with chronic periodontitis.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> GCF samples were collected, and clinical parameters including probing depth (PD), clinical attachment level, gingival index (GI), and plaque index were recorded. Thirty chronic periodontitis patients were randomized either to a low\u2010dose doxycycline (LDD) or placebo group. The LDD group received lowdose doxycycline (20 mg) b.i.d. for 3 months plus scaling and root planing (SRP), while the placebo group was given placebo capsules b.i.d. for 3 months plus SRP. The patients were evaluated every 3 months during the 12\u2010month study period. At each visit, all clinical measurements and GCF sampling were repeated. GCF MMP\u20108 levels were determined by a time\u2010resolved immunofluorescence assay. Intragroup comparisons were tested by the Friedman test followed by Wilcoxon signed\u2010rank test to analyze significance of changes over time. The Mann\u2010Whitney test was used to determine differences between the LDD and placebo groups.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Significant improvements were observed in all clinical parameters in both groups over the 12\u2010month period (<jats:italic>P<\/jats:italic> &lt;0.0125). The LDD group showed a significantly greater reduction in mean PD scores at 9 and 12 months and in mean GI scores at all time points than the placebo group (<jats:italic>P<\/jats:italic> &lt;0.05). From baseline to 12 months, GCF MMP\u20108 levels were significantly reduced in both groups (<jats:italic>P<\/jats:italic> &lt;0.0125). The GCF MMP\u20108 level in the LDD group was significantly lower than that of the placebo group at 6 months (<jats:italic>P<\/jats:italic> &lt;0.05).<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> The present results indicate that low\u2010dose doxycycline therapy in combination with scaling and root planing can reduce GCF MMP\u20108 levels and improve clinical periodontal parameters in patients with chronic periodontitis. These results provide additional information about the usefulness of low\u2010dose doxycycline therapy as an adjunct to non\u2010surgical periodontal therapy in the long\u2010term management of periodontal disease. The effectiveness and course of low\u2010dose doxycycline therapy can be monitored conveniently by assessing GCF MMP\u20108 levels. <jats:italic>J Periodontol 2004;75:106\u2010115<\/jats:italic>.<\/jats:p>","DOI":"10.1902\/jop.2004.75.1.106","type":"journal-article","created":{"date-parts":[[2005,2,2]],"date-time":"2005-02-02T07:48:21Z","timestamp":1107330501000},"page":"106-115","source":"Crossref","is-referenced-by-count":98,"title":["The Effect of Adjunctive Low\u2010Dose Doxycycline Therapy on Clinical Parameters and Gingival Crevicular Fluid Matrix Metalloproteinase\u20108 Levels in Chronic Periodontitis"],"prefix":"10.1002","volume":"75","author":[{"given":"G\u00fclnur","family":"Emingil","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00fcl","family":"Atilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timo","family":"Sorsa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanne","family":"Luoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Levent","family":"Kirilmaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haluk","family":"Baylas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,1]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2004.75.1.106","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T13:16:17Z","timestamp":1760879777000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2004.75.1.106"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2004,1]]}},"alternative-id":["10.1902\/jop.2004.75.1.106"],"URL":"https:\/\/doi.org\/10.1902\/jop.2004.75.1.106","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,1]]}}}