{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T22:18:46Z","timestamp":1791497926741,"version":"4.3.4"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,1,1]],"date-time":"2004-01-01T00:00:00Z","timestamp":1072915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Sera from patients with periodontal attachment loss contain higher concentrations of IgG anti\u2010phosphorylcholine (anti\u2010PC) than sera from healthy subjects. Furthermore, a large proportion of plaque bacteria bear PC\u2010containing surface antigens, implicating the oral flora as a source of immunogen for anti\u2010PC. Additionally, anti\u2010PC is cross\u2010reactive with a variety of oral bacterial antigens and human antigens such as oxidized low\u2010density lipoprotein (oxLDL). We hypothesized that, if the oral flora is a source of PC antigens, then we should be able to detect local anti\u2010 PC and anti\u2010oxLDL production in gingival crevicular fluid (GCF).<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> To test this, we collected 66 GCF samples from 15 patients with aggressive periodontitis and examined both the GCF samples and serum samples for their content of IgG anti\u2010PC, IgG anti\u2010LDL, and IgG anti\u2010oxLDL by enzyme\u2010linked immunosorbent assay. We also determined levels of anti\u2010tetanus toxoid (anti\u2010TT) as a non\u2010oral antigen control. Serum and GCF concentrations of serum albumin (HSA) were also determined for use as a dilution marker. A conservative GCF:serum antibody ratio of greater than 1.5 was considered to be evidence of local antibody production.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> For the non\u2010oral antigen TT, only one out of 62 samples contained locally produced antibody. Eight out of 64 samples (7 from a single subject) demonstrated local production of anti\u2010LDL. In contrast, 28 out of 66 samples demonstrated local production of anti\u2010PC, and 47 out of 66 samples contained locally produced anti\u2010oxLDL. It was observed that<jats:italic>A. actinomycetemcomitans<\/jats:italic> strains containing or devoid of PC could absorb antioxLDL from human sera. Although there was a correlation between the ratios of anti\u2010PC and anti\u2010oxLDL (Spearman's rho = 0.35, <jats:italic>P<\/jats:italic> = 0.0037), local production of both antibodies was found in only 17 out of 65 samples, indicating that these antibodies are not always reflective of reactivity to the same antigens.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> The local production of anti\u2010PC and anti\u2010oxLDL further implicates the oral flora as a source of antigen that may mediate immune reactions of relevance to cardiovascular and other systemic diseases. <jats:italic>J Periodontol 2004;75:146\u2010153<\/jats:italic>.<\/jats:p>","DOI":"10.1902\/jop.2004.75.1.146","type":"journal-article","created":{"date-parts":[[2005,2,2]],"date-time":"2005-02-02T07:48:21Z","timestamp":1107330501000},"page":"146-153","source":"Crossref","is-referenced-by-count":24,"title":["Locally Produced Anti\u2010Phosphorylcholine and Anti\u2010Oxidized Low\u2010Density Lipoprotein Antibodies in Gingival Crevicular Fluid from Aggressive Periodontitis Patients"],"prefix":"10.1002","volume":"75","author":[{"given":"Harvey A.","family":"Schenkein","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. Rennie","family":"Berry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John A.","family":"Burmeister","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carol N.","family":"Brooks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Al M.","family":"Best","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John G.","family":"Tew","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,1]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2004.75.1.146","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T13:16:18Z","timestamp":1760879778000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2004.75.1.146"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2004,1]]}},"alternative-id":["10.1902\/jop.2004.75.1.146"],"URL":"https:\/\/doi.org\/10.1902\/jop.2004.75.1.146","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,1]]}}}