{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T22:36:37Z","timestamp":1772058997482,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2004,10,1]],"date-time":"2004-10-01T00:00:00Z","timestamp":1096588800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2004,10]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Bone morphogenetic proteins (BMPs) are being evaluated for periodontal and bone regenerative therapy. The objective of this study was to evaluate the effect of recombinant human bone morphogenetic protein\u20104 (rhBMP\u20104) dose on local bone formation in a rat calvaria defect model.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Calvarial, 8 mm diameter, critical\u2010size osteotomy defects were created in 140 male Sprague\u2010Dawley rats. Seven groups of 20 animals each received either 1) rhBMP\u20104 (2.5 \u00b5g) in an absorbable collagen sponge (ACS) carrier, 2) rhBMP\u20104 (5 \u00b5g)\/ACS, 3) rhBMP\u20104 (2.5 \u00b5g) in a \u03b2\u2010tricalcium phosphate (\u03b2\u2010TCP) carrier, 4) rhBMP\u20104 (5 \u00b5g)\/\u03b2\u2010TCP, 5) ACS or 6) \u03b2\u2010TCP carrier controls, or 7) a sham\u2010surgery control, and were evaluated by histologic and histometric parameters following a 2\u2010 or 8\u2010week healing interval (10 animals\/group\/healing interval).<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Surgical implantation of rhBMP\u20104\/ACS and rhBMP\u2010 4\/\u03b2\u2010TCP resulted in enhanced local bone formation at both 2 and 8 weeks. Within the dose range examined, rhBMP\u20104 did not exhibit an appreciable dose\u2010dependent response. Defect closure was not significantly different between the rhBMP\u20104\/ACS and rhBMP\u20104\/\u03b2\u2010TCP groups. New bone area of the rhBMP\u20104\/ \u03b2\u2010TCP group was significantly greater than that of the rhBMP\u20104\/ ACS group; however, bone density in the rhBMP\u20104\/ACS group was significantly greater than that in the rhBMP\u20104\/\u03b2\u2010TCP group at 8 weeks (<jats:italic>P<\/jats:italic> &lt;0.05).<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> rhBMP\u20104 combined with ACS or \u03b2\u2010TCP has a significant potential to induce bone formation in the rat calvaria defect model. Within the selected rhBMP\u20104 dose range and observation interval, there appeared to be no meaningful differences in bone formation. <jats:italic>J Periodontol 2004;75:1364\u20101370<\/jats:italic>.<\/jats:p>","DOI":"10.1902\/jop.2004.75.10.1364","type":"journal-article","created":{"date-parts":[[2005,2,17]],"date-time":"2005-02-17T11:00:38Z","timestamp":1108638038000},"page":"1364-1370","source":"Crossref","is-referenced-by-count":60,"title":["Effect of Recombinant Human Bone Morphogenetic Protein\u20104 Dose on Bone Formation in a Rat Calvarial Defect Model"],"prefix":"10.1002","volume":"75","author":[{"given":"Eun\u2010Kyoung","family":"Pang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Se\u2010Ung","family":"Im","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang\u2010Sung","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seong\u2010Ho","family":"Choi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jung\u2010Kiu","family":"Chai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chong\u2010Kwan","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soo\u2010Boo","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyoo\u2010Sung","family":"Cho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2004.75.10.1364","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T11:20:21Z","timestamp":1760872821000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2004.75.10.1364"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,10]]},"references-count":0,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2004,10]]}},"alternative-id":["10.1902\/jop.2004.75.10.1364"],"URL":"https:\/\/doi.org\/10.1902\/jop.2004.75.10.1364","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,10]]}}}