{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,22]],"date-time":"2025-11-22T19:24:24Z","timestamp":1763839464683,"version":"build-2065373602"},"reference-count":0,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2004,12,1]],"date-time":"2004-12-01T00:00:00Z","timestamp":1101859200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> The aim of this study was to evaluate the effect of the association of locally delivered doxycycline 10% and scaling and root planing in the subgingival plaque of smokers.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Sixteen smokers with chronic periodontitis and a minimum of four pockets \u22655 mm) on anterior teeth that bled on probing were selected. Patients were randomly assigned to one of the following groups: scaling and root planing (SRP) or scaling and root planing followed by local application of doxycycline (SRP\u2010D). Subgingival plaque samples were collected from initially moderate (5 to 6 mm) and deep (\u22657 mm) pockets at baseline and 3 months. Polymerase chain reaction (PCR) analysis was used to detect the frequency of <jats:italic>Actinobacillus actinomycetemcomitans<\/jats:italic> (<jats:italic>Aa<\/jats:italic>), <jats:italic>Porphyromonas gingivalis<\/jats:italic> (<jats:italic>Pg<\/jats:italic>), and <jats:italic>Tannerella forsythensis<\/jats:italic> (<jats:italic>Tf<\/jats:italic>).<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> No statistically significant difference was found in the reduction of <jats:italic>Aa<\/jats:italic> in either the SRP\u2010D or SRP group (<jats:italic>P<\/jats:italic> &gt;0.05). The reduction in <jats:italic>Tf, Pg<\/jats:italic>, and <jats:italic>Tf + Pg<\/jats:italic> was statistically significant for SRP\u2010D only (<jats:italic>P<\/jats:italic> = 0.016, 0.027, and 0.027, respectively). The proportion of sites free of <jats:italic>Tf<\/jats:italic> at 3 months was 53% for SRP\u2010D and 9% for SRP (<jats:italic>P<\/jats:italic> = 0.02). For <jats:italic>Pg<\/jats:italic>, this proportion was 82% and 40%, respectively (<jats:italic>P<\/jats:italic> = 0.05).<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> The use of locally delivered doxycycline may promote the elimination of <jats:italic>T. forsythensis<\/jats:italic> and <jats:italic>P. gingivalis<\/jats:italic> in a greater proportion of sites compared to conventional scaling and root planing in smokers. <jats:italic>J Periodontol 2004;75:1600\u20101604<\/jats:italic>.<\/jats:p>","DOI":"10.1902\/jop.2004.75.12.1600","type":"journal-article","created":{"date-parts":[[2005,2,23]],"date-time":"2005-02-23T16:06:20Z","timestamp":1109174780000},"page":"1600-1604","source":"Crossref","is-referenced-by-count":16,"title":["Microbiological Changes With the Use of Locally Delivered Doxycycline in the Periodontal Treatment of Smokers"],"prefix":"10.1002","volume":"75","author":[{"given":"Luciana","family":"Machion","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denise Carleto","family":"Andia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Saito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marlize In\u00eas","family":"Klein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reginaldo Bruno","family":"Gon\u00e7alves","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1rcio Zafallon","family":"Casati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Francisco H.","family":"Nociti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enilson Antonio","family":"Sallum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,12]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2004.75.12.1600","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T10:04:13Z","timestamp":1760868253000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2004.75.12.1600"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,12]]},"references-count":0,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2004,12]]}},"alternative-id":["10.1902\/jop.2004.75.12.1600"],"URL":"https:\/\/doi.org\/10.1902\/jop.2004.75.12.1600","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"type":"print","value":"0022-3492"},{"type":"electronic","value":"1943-3670"}],"subject":[],"published":{"date-parts":[[2004,12]]}}}