{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T21:47:25Z","timestamp":1761947245393,"version":"build-2065373602"},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,3,1]],"date-time":"2004-03-01T00:00:00Z","timestamp":1078099200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2004,3]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Hereditary gingival fibromatosis (HGF) is a fibrotic gingival enlargement. In previous work, HGF fibroblasts grew faster and produced more collagen and fibronectin (FN) than normal gingival (GN) fibroblasts. HGF FN and collagen production, but not proliferation, were under autocrine transforming growth factor (TGF)\u2010\u03b2 control, suggesting other means of activation of HGF proliferation. Elevated\/prolonged expression of the protooncogene c\u2010<jats:italic>myc<\/jats:italic> is implicated in disregulation of cell growth. The objectives of this study were to: 1) determine if c\u2010<jats:italic>myc<\/jats:italic> expression is abnormal in quiescent and serum\u2010stimulated HGF and GN fibroblasts and 2) determine the relationship between c\u2010<jats:italic>myc<\/jats:italic> expression and fibroblast proliferation using a c\u2010<jats:italic>myc<\/jats:italic> antisense oligonucleotide (ODN).<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Proliferation was determined by enzyme\u2010linked immunosorbent assay (ELISA), measuring incorporation of bromodeoxyuridine into DNA. Expression of c\u2010<jats:italic>myc<\/jats:italic> was determined by quantitative polymerase chain reaction (PCR), using incorporation of fluorescent dCTP and detection via electrophoresis.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Proliferation was minimal until 24 hours or more after serum stimulation, when HGF proliferation was greater than GN (<jats:italic>P<\/jats:italic> \u22640.02). All cells expressed c\u2010<jats:italic>myc<\/jats:italic> mRNA at quiescence and \u22651 hour after serum stimulation. Expression of c\u2010<jats:italic>myc<\/jats:italic> in quiescent HGF fibroblasts was elevated, and it peaked and remained higher after serum stimulation than in GN cells. Proliferation of an HGF cell line was inhibited by 4 \u03bcM c\u2010<jats:italic>myc<\/jats:italic> antisense ODN (14% decrease; <jats:italic>P<\/jats:italic> \u22640.006) and 8 \u03bcM c\u2010<jats:italic>myc<\/jats:italic> antisense ODN (~80% decrease; <jats:italic>P<\/jats:italic> \u22640.0001), but generally not by c\u2010<jats:italic>myc<\/jats:italic> sense ODN. This effect was reversed by hybridizing the c\u2010<jats:italic>myc<\/jats:italic> antisense and sense ODNs (<jats:italic>P<\/jats:italic> = 0.007).<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> Data suggest that elevated proliferation of an HGF fibroblast cell line is related to elevated c\u2010<jats:italic>myc<\/jats:italic> expression. <jats:italic>J Periodontol 2004;75:360\u2010369<\/jats:italic>.<\/jats:p>","DOI":"10.1902\/jop.2004.75.3.360","type":"journal-article","created":{"date-parts":[[2005,2,2]],"date-time":"2005-02-02T07:52:16Z","timestamp":1107330736000},"page":"360-369","source":"Crossref","is-referenced-by-count":9,"title":["Role of the c\u2010<i>myc<\/i> Proto\u2010Oncogene in the Proliferation of Hereditary Gingival Fibromatosis Fibroblasts"],"prefix":"10.1002","volume":"75","author":[{"given":"David A.","family":"Tipton","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"III","given":"Ernest S.","family":"Woodard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark A.","family":"Baber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mustafa Kh.","family":"Dabbous","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,3]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2004.75.3.360","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T11:35:20Z","timestamp":1760873720000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2004.75.3.360"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,3]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2004,3]]}},"alternative-id":["10.1902\/jop.2004.75.3.360"],"URL":"https:\/\/doi.org\/10.1902\/jop.2004.75.3.360","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"type":"print","value":"0022-3492"},{"type":"electronic","value":"1943-3670"}],"subject":[],"published":{"date-parts":[[2004,3]]}}}