{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:39:26Z","timestamp":1760920766223,"version":"build-2065373602"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T00:00:00Z","timestamp":1104537600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> The purpose of this study was to determine whether lymphocytic cells regulate the monocytic hyperinflammatory trait (MO<jats:sup>+<\/jats:sup>) in chronic periodontitis patients. Using a <jats:italic>P. gingivalis<\/jats:italic> challenge model in severe combined immunodeficient (SCID) mice, we tested the effects of adoptively transferred human peripheral blood leukocytes from gingivitis and chronic periodontitis diabetic and non\u2010diabetic individuals on monocytic responses.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> This response was examined using the subcutaneous tissue chamber infection model. Three weeks following cell reconstitution, all SCID mice were challenged with 10<jats:sup>9<\/jats:sup> colony forming units of live <jats:italic>P. gingivalis<\/jats:italic> HG405. Chamber contents were collected at day 7 after bacterial challenge for prostaglandin E<jats:sub>2<\/jats:sub> (PGE<jats:sub>2<\/jats:sub>) analysis and chamber rejection monitored up to day 30. Gingival crevicular fluid (GCF) samples were collected from all patients for PGE<jats:sub>2<\/jats:sub> analysis. Both chamber fluid\u2010 and GCF\u2010PGE<jats:sub>2<\/jats:sub> levels were determined by enzyme\u2010linked immunosorbent assays (ELISA).<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Significantly elevated GCF\u2010PGE<jats:sub>2<\/jats:sub> levels were found in diabetic as well as in non\u2010diabetic patients with moderate\/advanced periodontitis compared to diabetic and non\u2010diabetic subjects with gingivitis\/mild periodontitis at <jats:italic>P<\/jats:italic> = 0.01 and <jats:italic>P<\/jats:italic> = 0.001, respectively. As reflected in chamber fluid PGE<jats:sub>2<\/jats:sub> levels and percentage chamber rejection, lymphocytic sensitization to <jats:italic>P. gingivalis<\/jats:italic> occurred in both diabetics and non\u2010diabetics with moderate\/advanced periodontitis, but not in diabetics and non\u2010diabetics with gingivitis\/mild periodontitis.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> These results suggest that the exaggerated monocytic inflammatory response trait (MO<jats:sup>+<\/jats:sup>) associated with moderate\/ advanced chronic periodontitis is due, at least in part, to lymphocytic modulation, while the directional findings for lymphocytes from diabetic subjects deserve further investigation. Our findings further demonstrate that the SCID mouse model is a useful animal model to study human immune responses to periodontal microorganisms. <jats:italic>J Periodontol 2005;76:16\u201021<\/jats:italic>.<\/jats:p>","DOI":"10.1902\/jop.2005.76.1.16","type":"journal-article","created":{"date-parts":[[2005,3,7]],"date-time":"2005-03-07T08:41:10Z","timestamp":1110184870000},"page":"16-21","source":"Crossref","is-referenced-by-count":4,"title":["Reconstitution of a Hyperinflammatory Prostaglandin E<sub>2<\/sub> Response to <i>Porphyromonas gingivalis<\/i> Challenge in Severe Combined Immunodeficient Mice"],"prefix":"10.1002","volume":"76","author":[{"given":"Giovanni E.","family":"Salvi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satu","family":"Spets\u2010Happonen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert E.","family":"Singer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven","family":"Offenbacher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,1]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2005.76.1.16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T12:52:32Z","timestamp":1760878352000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2005.76.1.16"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2005,1]]}},"alternative-id":["10.1902\/jop.2005.76.1.16"],"URL":"https:\/\/doi.org\/10.1902\/jop.2005.76.1.16","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"type":"print","value":"0022-3492"},{"type":"electronic","value":"1943-3670"}],"subject":[],"published":{"date-parts":[[2005,1]]}}}