{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T10:31:50Z","timestamp":1789813910262,"version":"4.0.1"},"reference-count":0,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2005,11,1]],"date-time":"2005-11-01T00:00:00Z","timestamp":1130803200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2005,11]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> This study aimed to investigate the interrelationship of in vivo expression of lipopolysaccharide\u2010binding protein (LBP) and membrane\u2010bound CD14 (mCD14) in human gingival tissues as well as the coexpression of Toll\u2010like receptors (TLR) 2 and 4 in association with periodontal conditions.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Gingival biopsies were collected from 43 subjects with chronic periodontitis, including periodontal pocket tissues (PoTs) and clinically healthy gingival tissues (HT\u2010Ps), and from 15 periodontally healthy subjects as controls (HT\u2010Cs). The expression of LBP, CD14, TLR 2, and TLR 4 was detected by immunohistochemistry and reverse transcription\u2010polymerase chain reaction (RT\u2010PCR).<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> LBP and mCD14 peptides were simultaneously detected in 91% of PoTs, 85% of HT\u2010Ps, and 100% of HT\u2010Cs. LBP and mCD14 mRNAs were simultaneously detected in 55% of PoTs, 55% of HT\u2010Ps, and 75% of HT\u2010Cs. The expression of LBP was confined to the gingival epithelium, whereas mCD14 was observed around the epithelium\u2010connective tissue interface. A positive correlation existed between LBP and mCD14 peptides in both detection expression (r<jats:sub>s<\/jats:sub> = 0.608; <jats:italic>P<\/jats:italic> &lt;0.001) and expression levels (r = 0.304; <jats:italic>P<\/jats:italic> &lt;0.05) of these two molecules. In PoTs, TLR 2 was detected in both pocket epithelia and macrophage\u2010like cells in connective tissues, whereas TLR 4 was predominantly detected in connective tissues. In HT\u2010Ps and HT\u2010Cs, a weak expression of TLR 2 was found in gingival epithelia, and no TLR 4 expression was detected. In PoTs, mCD14 was codetected on CD68\u2010labeled macrophages in the underlying connective tissues of pocket epithelium as well as on CD1a\u2010labeled dendritic cells in the pocket epithelium and connective tissues interface. No similar expression profile was detected in HT\u2010Ps and HT\u2010Cs.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> This study suggests that the in vivo expression of LBP and mCD14 may be interrelated. Altered cellular expression profiles of mCD14 and TLR 2 and 4 in periodontal pocket tissues imply that these pattern recognition receptors may play a role in periodontal pathogenesis.<\/jats:p>","DOI":"10.1902\/jop.2005.76.11.1950","type":"journal-article","created":{"date-parts":[[2005,11,7]],"date-time":"2005-11-07T13:20:15Z","timestamp":1131369615000},"page":"1950-1959","source":"Crossref","is-referenced-by-count":102,"title":["The Expression Profile of Lipopolysaccharide\u2010Binding Protein, Membrane\u2010Bound CD14, and Toll\u2010Like Receptors 2 and 4 in Chronic Periodontitis"],"prefix":"10.1002","volume":"76","author":[{"given":"Lei","family":"Ren","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. Keung","family":"Leung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard P.","family":"Darveau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijian","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,11]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2005.76.11.1950","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T12:08:11Z","timestamp":1760875691000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2005.76.11.1950"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,11]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2005,11]]}},"alternative-id":["10.1902\/jop.2005.76.11.1950"],"URL":"https:\/\/doi.org\/10.1902\/jop.2005.76.11.1950","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,11]]}}}