{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T16:52:53Z","timestamp":1777999973394,"version":"3.51.4"},"reference-count":0,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2005,12,1]],"date-time":"2005-12-01T00:00:00Z","timestamp":1133395200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2005,12]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> To examine the effects of cyclosporin A (CsA) on the expression of growth factors in induced gingival overgrowth with limited contributing factors arising from local inflammation caused by bacterial plaque, this study of gingival overgrowth was designed on the edentulous ridge of rats.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> After a 3\u2010week healing period following maxillary molar extractions, 16 five\u2010week\u2010old male Sprague\u2010Dawley rats were assigned to CsA and control groups. Animals in the CsA group were fed 30 mg\/kg CsA daily, whereas the control rats received a mineral oil vehicle instead. After 4 weeks, all animals were sacrificed, and the morphology of edentulous ridges was recorded by dental impression. The gingivae on the left\u2010hand side were dissected and stored for mRNA analysis, whereas the gingivae on the right\u2010hand side were fixed in 4% paraformaldehyde for immunohistochemistry (IHC) analysis of transforming growth factor\u2010\u03b21 (TGF\u2010\u03b21), platelet\u2010derived growth factor \u03b2 (PDGF\u2010\u03b2), insulin\u2010like growth factor\u20101 (IGF\u20101), and vascular endothelial growth factor (VEGF).<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> The edentulous gingivae were enlarged and the body weights were reduced in the CsA\u2010treated animals compared to controls. The mRNA expressions of TGF\u2010\u03b21, IGF\u20101, and VEGF were higher in the gingivae of the CsA group than in the control group. In addition, a greater mRNA expression (7.21\u2010fold) of VEGF was demonstrated in the CsA group than in the control group by real\u2010time polymerase chain reaction (PCR). The percentages of cells staining positive for TGF\u2010\u03b21 and VEGF were significantly greater in the CsA rats than in the control rats.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> Greater mRNA expression and positive staining for TGF\u2010\u03b21 and VEGF were observed in the edentulous gingivae of rats that received CsA. Therefore, CsA may upregulate TGF\u2010\u03b21 and VEGF gene expression and protein secretion in CsA\u2010induced gingival overgrowth.<\/jats:p>","DOI":"10.1902\/jop.2005.76.12.2267","type":"journal-article","created":{"date-parts":[[2005,12,6]],"date-time":"2005-12-06T16:44:45Z","timestamp":1133887485000},"page":"2267-2275","source":"Crossref","is-referenced-by-count":26,"title":["Upregulation of Transforming Growth Factor\u2010\u03b21 and Vascular Endothelial Growth Factor Gene and Protein Expression in Cyclosporin\u2010Induced Overgrown Edentulous Gingiva in Rats"],"prefix":"10.1002","volume":"76","author":[{"given":"Yen\u2010Teen","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsiao\u2010Pei","family":"Tu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Tang","family":"Chin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E\u2010Chin","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng\u2010Yang","family":"Chiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ching\u2010Hwa","family":"Gau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Earl","family":"Fu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2005.76.12.2267","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T12:02:19Z","timestamp":1760875339000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2005.76.12.2267"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,12]]},"references-count":0,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2005,12]]}},"alternative-id":["10.1902\/jop.2005.76.12.2267"],"URL":"https:\/\/doi.org\/10.1902\/jop.2005.76.12.2267","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,12]]}}}