{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T21:52:37Z","timestamp":1784757157270,"version":"3.55.0"},"reference-count":0,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,5,1]],"date-time":"2005-05-01T00:00:00Z","timestamp":1114905600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2005,5]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Periodontitis has been linked to increased risk of cardiovascular diseases. Systemic reactions associated with cardiovascular events may depend on characteristics of the subgingival microflora in periodontitis. Our objectives were to compare the numbers of cultivable bacteria, composition of subgingival microflora and clonal distribution of <jats:italic>Actinobacillus actinomycetemcomitans<\/jats:italic> (<jats:italic>A. actinomycetemcomitans<\/jats:italic>) in two groups of patients with generalized chronic periodontitis (GCP), one with an acute myocardial infarction (AMI\u2010GCP) and the other one without AMI (non\u2013AMI\u2010GCP).<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> In all, 150 dentate individuals were screened for suitability to this study. Subgingival bacterial samples were collected from 11 AMI\u2010GCP and 11 non\u2013AMI\u2010GCP patients who had been selected using strict inclusion criteria in an attempt to exclude confounding factors and to increase comparability of periodontal conditions by matching for periodontal probing depths and attachment levels. Culture methods were used to determine the total viable counts and occurrence and proportions of six periodontal bacterial species and yeasts. Polymerase chain reaction (PCR) technique was used to detect <jats:italic>A. actinomycetemcomitans<\/jats:italic> and <jats:italic>Porphyromonas gingivalis<\/jats:italic> (<jats:italic>P. gingivalis<\/jats:italic>). Intraspecies characterization of <jats:italic>A. actinomycetemcomitans<\/jats:italic> included serotyping and genotyping.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> The mean proportions of <jats:italic>P. gingivalis<\/jats:italic> (<jats:italic>P<\/jats:italic> = 0.05) and <jats:italic>Tannerella forsythensis<\/jats:italic> (<jats:italic>T. forsythensis<\/jats:italic>) (<jats:italic>P<\/jats:italic> = 0.01) were significantly lower, but the numbers of <jats:italic>Micromonas micros<\/jats:italic> (<jats:italic>M. micros<\/jats:italic>) and <jats:italic>A. actinomycetemcomitans<\/jats:italic> were up to nine times higher and the mean total number of cultivable bacteria per sample higher (<jats:italic>P<\/jats:italic> &lt;0.01) in AMI\u2010GCP than in non\u2013AMI\u2010GCP.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> The findings that no target subgingival species were overrepresented but the total bacterial number was higher in AMI\u2010GCP than non\u2013AMI\u2010GCP patients may provide support to the hypothesis that elevated numbers of bacteria in close vicinity to sterile parenteral area present a risk for systemic health.<jats:italic>J Periodontol 2005;76:740\u2010748<\/jats:italic>.<\/jats:p>","DOI":"10.1902\/jop.2005.76.5.740","type":"journal-article","created":{"date-parts":[[2005,5,18]],"date-time":"2005-05-18T05:45:34Z","timestamp":1116395134000},"page":"740-748","source":"Crossref","is-referenced-by-count":16,"title":["Characteristics of Periodontal Microflora in Acute Myocardial Infarction"],"prefix":"10.1002","volume":"76","author":[{"given":"Ba\u015fak","family":"Do\u011dan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eralp","family":"Buduneli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00fclnur","family":"Emingil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00fcl","family":"Atilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azem","family":"Akilli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna","family":"Antinheimo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Lakio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sirkka","family":"Asikainen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,5]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2005.76.5.740","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T11:18:32Z","timestamp":1760872712000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2005.76.5.740"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,5]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2005,5]]}},"alternative-id":["10.1902\/jop.2005.76.5.740"],"URL":"https:\/\/doi.org\/10.1902\/jop.2005.76.5.740","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,5]]}}}