{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T00:46:22Z","timestamp":1778201182845,"version":"3.51.4"},"reference-count":16,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,2,1]],"date-time":"2006-02-01T00:00:00Z","timestamp":1138752000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Studies have demonstrated that high levels of collagenase activity in gingival crevicular fluid (GCF) are associated with degradation of periodontal tissues in progressive periodontitis compared to periodontally healthy tissues. Because the activation of collagenases is an important issue in periodontitis, we have studied the activation of collagenase in gingival crevicular fluid samples of diabetic patients.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Collagenase activity was studied in human gingival crevicular fluids. Twenty\u2010two poorly controlled diabetic patients (e.g., blood glucose: 11.0 \u00b1 0.7 mmol\/l; hemoglobin A<jats:sub>1c<\/jats:sub> [HbA<jats:sub>1c<\/jats:sub>]: 9.6% \u00b1 0.3%) and five well\u2010controlled diabetic patients were compared to six chronic periodontitis subjects and five healthy controls. Collagenase activity against type I collagen was measured using sodium dodecyl sulfate\u2010polyacrylamide gel electrophoresis analysis quantitated by laser densitometry.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> The poorly controlled diabetic patients had more alveolar bone loss than the well\u2010controlled diabetic subjects and controls (<jats:italic>P<\/jats:italic> &lt;0.001; <jats:italic>t<\/jats:italic> test). The activity of collagenases in GCF in poorly controlled diabetic patients was similar to that seen in chronic periodontitis subjects (<jats:italic>P<\/jats:italic> &gt;0.05) but higher than in healthy controls (<jats:italic>P<\/jats:italic> &lt;0.01; <jats:italic>t<\/jats:italic> test), whereas there was no difference between the well\u2010controlled diabetic subjects and systemically healthy controls (<jats:italic>P<\/jats:italic> &gt;0.05; <jats:italic>t<\/jats:italic> test).<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> Poorly controlled diabetes is strongly related to periodontal tissue destruction, and collagenases in GCF may mediate and reflect this effect.<\/jats:p>","DOI":"10.1902\/jop.2006.040322","type":"journal-article","created":{"date-parts":[[2006,2,6]],"date-time":"2006-02-06T14:53:47Z","timestamp":1139237627000},"page":"189-194","source":"Crossref","is-referenced-by-count":30,"title":["Collagenases in Gingival Crevicular Fluid in Type 1 Diabetes Mellitus"],"prefix":"10.1002","volume":"77","author":[{"given":"Bedia","family":"Safkan\u2010Sepp\u00e4l\u00e4","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timo","family":"Sorsa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taina","family":"Tervahartiala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arzu","family":"Beklen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yrj\u00f6 T.","family":"Konttinen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1993.tb00223.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1992.tb01144.x"},{"key":"e_1_2_7_4_1","unstructured":"2001 University of Helsinki Helsinki B Safkan\u2010Sepp\u00e4l\u00e4 Periodontal disease in insulin\u2010dependent diabetics. 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