{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T15:30:38Z","timestamp":1760887838507,"version":"build-2065373602"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,3,1]],"date-time":"2006-03-01T00:00:00Z","timestamp":1141171200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Hemagglutinin and outer membrane protein (OMP) are major virulence factors associated with colonization of <jats:italic>Porphyromonas gingivalis<\/jats:italic> in the gingival crevice. The genes for the 200\u2010kDa antigenic protein (200\u2010kDa AP) and 40\u2010kDa OMP of <jats:italic>P. gingivalis<\/jats:italic> have been successfully cloned. Additionally, the 200\u2010kDa AP gene has been shown to constitute the hemagglutinin A (hagA) gene of <jats:italic>P. gingivalis<\/jats:italic>. Therefore, this study was constructed to evaluate the distributions and serum levels of immnoglobulin G (IgG) antibodies specific for 200\u2010kDa AP and 40\u2010kDa OMP in periodontitis patients.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Fifty patients with chronic periodontitis and 59 controls without periodontal destruction were enrolled in this study. We cloned the genes for 200\u2010kDa AP and 40\u2010kDa OMP from <jats:italic>P. gingivalis<\/jats:italic> and constructed the purified recombinant proteins. Serum levels of IgG subclass antibodies specific for both recombinant 200\u2010kDa and 40\u2010kDa OMP were determined in patients and controls by an enzyme\u2010linked immunosorbent assay (ELISA).<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> The serum IgG subclass distribution for patients and controls was IgG1 &gt; IgG4 &gt; IgG2 &gt; IgG3 in the anti\u2010200\u2010kDa AP response, which was almost identical to that in the anti\u201040\u2010kDa OMP response. The patient group showed significantly higher serum IgG responses to the 40\u2010kDa OMP than the control group (<jats:italic>P<\/jats:italic> &lt;0.01). In contrast, IgG subclass responses to the 200\u2010kDa AP were not different between the patients and controls. Serum levels of antibodies reactive with both 200\u2010kDa and 40\u2010kDa proteins did not have a significant association with mean probing depth.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> These results suggested that serum IgG responses against <jats:italic>P. gingivalis<\/jats:italic> OMP rather than the hagA may be more active in chronic periodontitis.<\/jats:p>","DOI":"10.1902\/jop.2006.050138","type":"journal-article","created":{"date-parts":[[2006,3,2]],"date-time":"2006-03-02T15:32:59Z","timestamp":1141313579000},"page":"364-369","source":"Crossref","is-referenced-by-count":10,"title":["Antibody Responses to <i>Porphyromonas gingivalis<\/i> Hemagglutinin A and Outer Membrane Protein in Chronic 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