{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:37:05Z","timestamp":1760920625650,"version":"build-2065373602"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,4,1]],"date-time":"2006-04-01T00:00:00Z","timestamp":1143849600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2006,4]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Cultured gingival substitute has been found to be a useful graft material for treatment of gingival recession. However, such substitutes include xenograft derivative materials that involve concomitant risk of viral contamination. To eliminate this risk, we designed new gingival substitutes made of recombinant human collagen types I and III sponges and cultured these substitutes in animal\u2010free media (HFDM\u20101).<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Gingival fibroblasts were seeded onto sponges of type I or III recombinant collagen. These sponges were cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS), HFDM\u20101 with 2% human serum (HS), or HFDM\u20101. Fibroblast proliferation in these samples was compared using the cell\u2010counting kit assay. Vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) released into the cultured media were examined by enzyme\u2010linked immunosorbent assay.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> The fibroblasts proliferated significantly in all six combinations of collagen and medium types. The fibroblast growth rate after 9 days of culture was equal between HFDM\u20101 with 2% HS and DMEM with 10% FBS. The type III collagen sponge showed a higher fibroblast growth rate than the type I sponge. VEGF concentrations in HFDM\u20101 with 2% HS were higher than those in other media. The highest HGF levels were detected in DMEM with 10% FBS.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> The new cultured gingival substitute containing no animal\u2010derived materials produced good cell proliferation and VEGF release. The results suggested that the substitute may provide a new tool for the treatment of gingival recession.<\/jats:p>","DOI":"10.1902\/jop.2006.050223","type":"journal-article","created":{"date-parts":[[2006,4,3]],"date-time":"2006-04-03T12:17:31Z","timestamp":1144066651000},"page":"672-677","source":"Crossref","is-referenced-by-count":23,"title":["Fabrication of Cultured Oral Gingiva by Tissue Engineering Techniques Without Materials of Animal Origin"],"prefix":"10.1002","volume":"77","author":[{"given":"Kazuto","family":"Yamada","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junji","family":"Yamaura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masakazu","family":"Katoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken\u2010ichiro","family":"Hata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiro","family":"Okuda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiromasa","family":"Yoshie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8493529"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.1995.1.3"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.3.1274"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.66.1.51"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.79.1.67"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1042\/BA20030229"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0094-1298(03)00075-0"},{"key":"e_1_2_8_9_1","first-page":"42","article-title":"New cell\u2010based technologies in bone and cartilage tissue engineering. 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