{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T16:52:53Z","timestamp":1777999973177,"version":"3.51.4"},"reference-count":55,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,5,1]],"date-time":"2006-05-01T00:00:00Z","timestamp":1146441600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> To examine the role of E\u2010cadherin in epithelial hyperplasia of cyclosporin A (CsA)\u2010induced gingival enlargement, mRNA and protein levels of E\u2010cadherin, \u03b2\u2010catenin, proliferating cell nuclear antigen (PCNA), and Cyclin D1 were examined in the edentulous gingiva of rats following CsA treatment.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Three weeks after the extraction of all maxillary molars, 20 male Sprague\u2010Dawley rats were assigned to a CsA\u2010fed group (30 mg\/kg daily) or a control group. Five rats per group were sacrificed at weeks 1 and 4. Edentulous ridge specimens were taken, and the expression levels of E\u2010cadherin, \u03b2\u2010catenin, Cyclin D1, and PCNA mRNAs were estimated by reverse transcription\u2010polymerase chain reaction (RT\u2010PCR). Tissue specimens of the week 4 groups were examined using immunohistochemical (IHC) staining for proteins.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> The mRNA expression of E\u2010cadherin was significantly weaker in the CsA\u2010treated group than the control group at both times. Using IHC staining, a weaker level of membrane\u2010bonded E\u2010cadherin was also observed in the gingival epithelial cells in the CsA group than in controls. By contrast, significantly stronger \u03b2\u2010catenin and Cyclin D1 mRNA expressions and protein levels were found in CsA\u2010treated rats than controls by RT\u2010PCR and immunohistochemistry at week 4, whereas PCNA production was stronger at both times.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> CsA treatment reduced the production of E\u2010cadherin but increased the production of \u03b2\u2010catenin, Cyclin D1, and PCNA. Thus, CsA may downregulate E\u2010cadherin gene expression, leading to the epithelial cell proliferation of gingival overgrowth.<\/jats:p>","DOI":"10.1902\/jop.2006.050316","type":"journal-article","created":{"date-parts":[[2006,5,3]],"date-time":"2006-05-03T23:25:50Z","timestamp":1146698750000},"page":"832-839","source":"Crossref","is-referenced-by-count":18,"title":["Cyclosporin\u2010Induced Downregulation of the Expression of E\u2010Cadherin During Proliferation of Edentulous Gingival Epithelium in Rats"],"prefix":"10.1002","volume":"77","author":[{"given":"Hsiao\u2010Pei","family":"Tu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yen\u2010Teen","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi\u2010Shing","family":"Shieh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ren\u2010Yeong","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Tang","family":"Chin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shih\u2010Fang","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ching\u2010Hwa","family":"Gau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Earl","family":"Fu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1983.tb01272.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1986.57.12.771"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0030-4220(83)90327-4"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1992.tb00098.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1994.65.6.565"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1989.tb00876.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240460209"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240440407"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051X.2003.00402.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2001.72.2.167"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2001.72.7.889"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2001.72.6.745"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2004.00223.x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0215429"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1094291"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/35000034"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/35000025"},{"key":"e_1_2_8_19_1","first-page":"1613","article-title":"The SLUG zinc\u2010finger protein represses E\u2010cadherin in breast cancer","volume":"62","author":"Hajra KM","year":"2002","journal-title":"Cancer Res"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0714.2002.00108.x"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1011319507155"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1992.63.6.548"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1991.tb01698.x"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.1996.tb00226.x"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1993.tb02113.x"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000027562.91075.56"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.11.8.2793"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200107065"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200104035"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90136-E"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.6.2444"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M303138200"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.105.4.1549"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2002.tb01339.x"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v9.i12.2863"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00012411"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.19.9.6379"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199911)181:2<258::AID-JCP8>3.0.CO;2-Q"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00405-002-0500-z"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200212033"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc822"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65575-7"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:JOMG.0000017430.12210.37"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2004.12.009"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.2214\/ajr.154.2.2105012"},{"key":"e_1_2_8_46_1","first-page":"313","article-title":"No difference in skin cancer incidence with or without cyclosporine \u2013 A 5\u2010year perspective","volume":"24","author":"Blohme I","year":"1992","journal-title":"Transplant Proc"},{"key":"e_1_2_8_47_1","first-page":"622","article-title":"Malignant tumors after heart transplantation","volume":"9","author":"Couetil JP","year":"1990","journal-title":"J Heart Transplant"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2004.12.008"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2005.03.002"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M413878200"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.3.5.885"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-008X(03)00170-4"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.942"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(03)00165-5"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-419X(03)00005-2"},{"key":"e_1_2_8_56_1","first-page":"5947","article-title":"PML is a target gene of beta\u2010catenin and plakoglobin, and coactivates beta\u2010catenin\u2010mediated transcription","volume":"62","author":"Shtutman M","year":"2002","journal-title":"Cancer Res"}],"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2006.050316","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T12:03:27Z","timestamp":1760875407000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2006.050316"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,5]]},"references-count":55,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2006,5]]}},"alternative-id":["10.1902\/jop.2006.050316"],"URL":"https:\/\/doi.org\/10.1902\/jop.2006.050316","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,5]]}}}