{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T17:13:13Z","timestamp":1768929193538,"version":"3.49.0"},"reference-count":50,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2006,11,1]],"date-time":"2006-11-01T00:00:00Z","timestamp":1162339200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Matrix metalloproteinases (MMPs) participate in extracellular matrix degradation in physiological and pathological conditions. The available evidence suggests that MMP\u201013 plays a significant role in both the initiation and progress of bone resorption. The aim of our study was to identify the presence of MMP\u201013 in adult patients with untreated chronic periodontitis. We also determined the activity of MMP\u201013 present in lesions undergoing episodic attachment loss in gingival crevicular fluid (GCF) samples.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> After monitoring at 2 and 4 months, 21 patients showed destructive periodontitis (periodontally affected sites presenting at least two sites with \u22652 mm clinical attachment loss), and GCF samples were collected both from active and inactive sites (21 GCF samples, each). GCF was collected during a 30\u2010second interval using a paper strip, and an immunofluorescence assay was performed to determine the basal activity of MMP\u201013 and the relationship between 4\u2010aminophenylmercuric acetate (APMA)\u2010activated total MMP\u201013 and basal MMP\u201013 activity. Gingival tissues from five patients were fixed in formalin and MMP\u201013 expression was demonstrated using immunohistochemistry and <jats:italic>in situ<\/jats:italic> hybridization. MMP\u201013 molecular forms were examined by Western immunoblotting with monoclonal antibodies.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> MMP\u201013 was found in 100% of GCF samples from patients with chronic periodontitis. Active sites, associated with tissue destruction, had significantly higher proportions of active MMP\u201013 and MMP\u201013 activity levels than their inactive counterparts (1.49 versus 1.17 ng fluorescent product, respectively; <jats:italic>P<\/jats:italic> &lt;0.05). Western blot, immunohistochemical staining, and <jats:italic>in situ<\/jats:italic> hybridization confirmed the presence of MMP\u201013 in periodontal disease, with observable differences between periodontitis and healthy subjects. MMP\u201013 immunoreactivities were seen mainly as 55 and 48 kDa, corresponding to partially and fully activated forms, respectively, and a smaller proportion of 60\u2010kDa proenzyme form.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> MMP\u201013 activity in GCF samples was significantly increased in active sites from progressive periodontal disease, supporting its role in the alveolar bone loss developed in this disease.<\/jats:p>","DOI":"10.1902\/jop.2006.050461","type":"journal-article","created":{"date-parts":[[2006,10,31]],"date-time":"2006-10-31T12:54:05Z","timestamp":1162299245000},"page":"1863-1870","source":"Crossref","is-referenced-by-count":93,"title":["Matrix Metalloproteinase\u201013 Is Highly Expressed in Destructive Periodontal Disease Activity"],"prefix":"10.1002","volume":"77","author":[{"given":"Marcela","family":"Hernandez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Antonieta","family":"Valenzuela","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Lopez\u2010Otin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jes\u00fas","family":"Alvarez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose Manuel","family":"Lopez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rolando","family":"Vernal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Gamonal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1999.4.1.1"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1986.tb01485.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0501.2003.140302.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345970760060501"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.9.6059"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0757.2000.2240111.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0765.2002.00365.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0765.2002.00663.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1994.tb00272.x"},{"key":"e_1_2_8_11_1","first-page":"342","article-title":"Inmunohistochemical study of neutrophil\u2010 and fibroblast\u2010type collagenases and stromelysin\u20101 in adult periodontitis","volume":"102","author":"Ingman T","year":"1994","journal-title":"Scand J Dent Res"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1994.tb01100.x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s000110050193"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.1998.tb00286.x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1997.tb00195.x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0757.2003.03106.x"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.041811af"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.108.2.529"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.108.6.2221"},{"key":"e_1_2_8_20_1","first-page":"717","article-title":"Collagenase\u20103 (MMP\u201013) is expressed during human fetal ossification and re\u2010expressed in postnatal bone remodeling and in rheumatoid arthritis","volume":"76","author":"St\u00e4hle\u2010Backdahl M","year":"1997","journal-title":"Lab Invest"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345000790120801"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.52.37292"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1983.tb01278.x"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.110.2.609"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2000.71.10.1535"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar572"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118475"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI10564"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar600"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.3.1544"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1991.62.3.185"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2005.00117.x"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1990.61.7.420"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051x.2002.290308.x"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1999.70.11.1292"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.52.37292"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1177\/08959374980120010501"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2528207"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1679\/aohc.57.535"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.1013"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.124.6.1091"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.151.4.879"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)89457-7"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.108.6.2221"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1177\/002215540205000304"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s007740200020"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2003.09.001"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1982.tb02046.x"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2002.17.1.77"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(95)01539-6"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1997.00369.x"}],"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2006.050461","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T13:36:04Z","timestamp":1760880964000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2006.050461"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,11]]},"references-count":50,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2006,11]]}},"alternative-id":["10.1902\/jop.2006.050461"],"URL":"https:\/\/doi.org\/10.1902\/jop.2006.050461","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,11]]}}}