{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T02:40:50Z","timestamp":1791081650502,"version":"4.1.0"},"reference-count":64,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2006,12,1]],"date-time":"2006-12-01T00:00:00Z","timestamp":1164931200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> During periodontal inflammation, matrix metalloproteinases (MMPs) are under the control of several regulatory mechanisms including the upregulation of expression by inducers and downregulation by inhibitors. Our study aimed to examine the levels and molecular forms of MMP\u20107, tissue inhibitor of MMP (TIMP)\u20101, and extracellular matrix metalloproteinase inducer (EMMPRIN) in gingival crevicular fluid (GCF) from patients with different periodontal diseases.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> A total of 80 subjects (20 patients with generalized aggressive periodontitis [GAgP], 20 with chronic periodontitis [CP], 20 with gingivitis, and 20 periodontally healthy subjects) were included in this study. Periodontal status was evaluated by measuring probing depth, clinical attachment loss, presence of bleeding on probing, and plaque. GCF MMP\u20107, TIMP\u20101, and EMMPRIN levels and molecular forms were analyzed by enzyme\u2010linked immunosorbent assay (ELISA) and Western immunoblot techniques using specific antibodies.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Total amounts of GCF MMP\u20107 were found to be similar between the study groups. GAgP, CP, and gingivitis groups had significantly higher total amounts of GCF EMMPRIN compared to healthy subjects (<jats:italic>P<\/jats:italic> &lt;0.008). Among the patient groups, the GAgP group had the highest total amount of GCF EMMPRIN relative to the gingivitis group (<jats:italic>P<\/jats:italic> = 0.0004). Soluble EMMPRIN existed in GCF in multiple molecular\u2010weight species especially in periodontitis\u2010affected GCF under non\u2010reducing conditions, i.e., 30\u2010, \u20105\u2010, 100\u2010, 180\u2010, and 200\u2010kDa species. All patient groups had significantly elevated total amounts of GCF TIMP\u20101 relative to the healthy group (<jats:italic>P<\/jats:italic> &lt;0.0001). GAgP and CP groups also had a higher total amount of GCF TIMP\u20101 compared to the gingivitis group (<jats:italic>P<\/jats:italic> &lt;0.0001 and <jats:italic>P<\/jats:italic> &lt;0.0001, respectively). The GAgP group had higher GCF TIMP\u20101 and EMMPRIN levels compared to the CP group, but this elevation did not reach statistical significance.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> Our data indicate that MMP\u20107 is associated with the innate host defense in periodontal tissues. Increased EMMPRIN and TIMP\u20101 levels in GCF are associated with the enhanced severity of periodontal inflammation, indicating that these molecules can participate in the regulation of progression of periodontal diseases. To our knowledge, the present study demonstrated the presence of soluble forms of EMMPRIN in GCF of patients with different periodontal diseases for the first time.<\/jats:p>","DOI":"10.1902\/jop.2006.060144","type":"journal-article","created":{"date-parts":[[2006,11,30]],"date-time":"2006-11-30T08:25:32Z","timestamp":1164875132000},"page":"2040-2050","source":"Crossref","is-referenced-by-count":52,"title":["Gingival Crevicular Fluid Matrix Metalloproteinase (MMP)\u20107, Extracellular MMP Inducer, and Tissue Inhibitor of MMP\u20101 Levels in Periodontal Disease"],"prefix":"10.1002","volume":"77","author":[{"given":"G\u00fclnur","family":"Emingil","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taina","family":"Tervahartiala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P\u00e3ivi","family":"M\u00e3ntyl\u00e3","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marko","family":"M\u00e4\u00e4tt\u00e4","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timo","family":"Sorsa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00fcl","family":"Atilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1997.tb00191.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2005.00140.x"},{"key":"e_1_2_7_4_1","first-page":"154","article-title":"The 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