{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T19:35:19Z","timestamp":1790019319644,"version":"4.0.1"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:00:00Z","timestamp":1172707200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Compressive force is an important mechanical stimulus on the periodontal ligament (PDL) and is closely related to therapeutic tooth movement. In this study, early or late response genes related to the compressive stress in PDL cells were evaluated. Particularly, the expression of interleukin (IL)\u20106, IL\u20108, and alkaline phosphatase (ALP) was studied.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> The primary cultured cells from PDL were grown in a three\u2010dimensional collagen gel, and received a continuous static compressive force (1.76 g\/cm<jats:sup>2<\/jats:sup>). The expressed genes were screened by cDNA microarray assays for 2 or 12 hours after the initiation of the mechanical force application. The genes of interest that showed significant changes in expression in the cDNA microarray assay were analyzed further by quantitative reverse transcriptase polymerase chain reaction (RT\u2010PCR), enzyme\u2010linked immunoabsorbent assays (ELISA), and ALP assays.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> ALP, IL\u20106, and IL\u20108 were selected among the genes that significantly changed expression (\/M\/ &gt;0.7) and subsequently were confirmed by quantitative RT\u2010PCR. The secreted protein concentrations for IL\u20106, IL\u20108, and ALP activity were measured at 72 hours after application of continuous static compressive force. The protein level of IL\u20106 was significantly increased at 72 hours (<jats:italic>P<\/jats:italic> &lt;0.001), but there was no significant change in IL\u20108 (<jats:italic>P<\/jats:italic> &gt;0.0\u2010). ALP activity was decreased \u223c41.5% compared to the control (<jats:italic>P<\/jats:italic> = 0.015).<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> Considering that IL\u20106 is a potent osteoclast activator and the compressive side of PDL during orthodontic tooth movement shows the resorption of calcified tissue, the changed expression of IL\u20106 and ALP in response to the static compressive force in PDL cells may contribute to the orthodontic tooth movement or alveolar bone remodeling.<\/jats:p>","DOI":"10.1902\/jop.2007.060240","type":"journal-article","created":{"date-parts":[[2007,3,2]],"date-time":"2007-03-02T14:08:16Z","timestamp":1172844496000},"page":"446-452","source":"Crossref","is-referenced-by-count":61,"title":["Differential Gene Expression of Periodontal Ligament Cells After Loading of Static Compressive Force"],"prefix":"10.1002","volume":"78","author":[{"given":"Yeon\u2010Hee","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong\u2010Seok","family":"Nahm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youn\u2010Kwan","family":"Jung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Je\u2010Yong","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sahng Gyoon","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Cho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myung\u2010Hee","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang\u2010Hoon","family":"Chae","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seong\u2010Gon","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2003.74.6.780"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/ejo\/12.1.51"},{"key":"e_1_2_7_4_1","first-page":"68","article-title":"An assessment of the control of matrix turnover by a quantitative ultrastructural analysis of fibroblasts of the periodontal ligament in rats","volume":"19","author":"Shore RC","year":"1991","journal-title":"J Biol Buccale"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1972.tb00630.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9416(69)90143-2"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9290(74)90086-4"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-3913(78)90031-8"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9290(76)90174-3"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(81)90132-1"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajodo.2004.03.012"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-3913(90)90238-8"},{"key":"e_1_2_7_13_1","first-page":"42","article-title":"Parameters of MOD cavity preparations: A 3\u2010D FEM study, Part II","volume":"16","author":"Khera SC","year":"1991","journal-title":"Oper Dent"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345880670110201"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0141-5425(90)90007-A"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9290(02)00166-5"},{"key":"e_1_2_7_17_1","doi-asserted-by":"crossref","unstructured":"Fung YC.Biomechanics: Mechanical Properties of Living Tissues. 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