{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T10:54:37Z","timestamp":1791888877166,"version":"4.3.4"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2007,6,1]],"date-time":"2007-06-01T00:00:00Z","timestamp":1180656000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Receptor activator of nuclear factor\u2010kappa B (NF\u2010\u03baB) ligand (RANKL) and osteoprotegerin (OPG) are critical for homeostatic control of osteoclast activity, suggesting their vital roles in the progression of bone loss in periodontitis. In this study, the expression of RANKL and OPG mRNA and the relationship between these factors and periodontopathic bacteria in periodontal tissue were studied.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Gingival tissue and subgingival plaque samples were collected from 15 patients with chronic periodontitis and 15 periodontally healthy subjects. RNA was extracted from the tissue and subjected to reverse transcription\u2010polymerase chain reaction (RT\u2010PCR) using primers specific for RANKL or OPG. \u03b2\u2010actin was amplified as a control to ensure equal loading. The intensity of RT\u2010PCR products was analyzed by a densitometer in proportion to the intensity of \u03b2\u2010actin. The numbers of <jats:italic>Porphyromonas gingivalis<\/jats:italic> and <jats:italic>Actinobacillus actinomycetemcomitans<\/jats:italic> were determined by quantitative real\u2010time PCR.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Our results showed increased levels of RANKL mRNA in chronic periodontitis tissues. The RANKL\/OPG expression ratio was significantly higher in the periodontitis group compared to the healthy control group (<jats:italic>P<\/jats:italic> = 0.001). Interestingly, the expression of RANKL (r = 0.64; <jats:italic>P<\/jats:italic> &lt;0.001), but not OPG (r = \u22120.24; <jats:italic>P<\/jats:italic> = 0.20), was significantly correlated with increased numbers of <jats:italic>P. gingivalis<\/jats:italic>. <jats:italic>A. actinomycetemcomitans<\/jats:italic> was detected in only 6.7% of all sites.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> Chronic periodontitis was associated with RANKL mRNA upregulation and increased RANKL\/OPG mRNA expression ratio. In addition, our data showed for the first time to our knowledge an association between upregulated RANKL levels and the number of <jats:italic>P. gingivalis<\/jats:italic> in clinically obtained periodontal tissues.<\/jats:p>","DOI":"10.1902\/jop.2007.060398","type":"journal-article","created":{"date-parts":[[2007,5,31]],"date-time":"2007-05-31T08:27:43Z","timestamp":1180600063000},"page":"1062-1069","source":"Crossref","is-referenced-by-count":59,"title":["RANKL Upregulation Associated With Periodontitis and <i>Porphyromonas gingivalis<\/i>"],"prefix":"10.1002","volume":"78","author":[{"given":"Nawarat","family":"Wara\u2010aswapati","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudee","family":"Surarit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anek","family":"Chayasadom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason A.","family":"Boch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waranuch","family":"Pitiphat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1996.1.1.879"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.7.3597"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/16852"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/edrv.20.3.0367"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/46303"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80209-3"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.139.3.5837"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.12.9.1260"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.8697"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI10763"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.5.3574"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.3.1706-1714.2004"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2004.75.12.1647"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1999.4.1.1"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0928-8244(03)00224-4"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1996.1.1.821"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345010800030801"},{"key":"e_1_2_8_19_1","first-page":"17","article-title":"Expression of RANKL and OPG mRNA in periodontal disease: Possible involvement in bone destruction","volume":"11","author":"Liu D","year":"2003","journal-title":"Int J Mol Med"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910408300216"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81569-X"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2001.16.6.1015"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2003.18.2.270"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1359\/JBMR.050503"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2006.060180"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2006.050376"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2006.00883.x"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.1999.14.9.1486"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.01.024"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2002.01990.x"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(99)00162-3"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2004.75.2.249"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200523394"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2004.09.011"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.5.813"}],"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2007.060398","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T13:38:10Z","timestamp":1760881090000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2007.060398"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,6]]},"references-count":34,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2007,6]]}},"alternative-id":["10.1902\/jop.2007.060398"],"URL":"https:\/\/doi.org\/10.1902\/jop.2007.060398","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,6]]}}}