{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:21:58Z","timestamp":1760919718954,"version":"build-2065373602"},"reference-count":19,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2007,12,1]],"date-time":"2007-12-01T00:00:00Z","timestamp":1196467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2007,12]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Leukotriene B<jats:sub>4<\/jats:sub> (LTB<jats:sub>4<\/jats:sub>) is a membrane\u2010derived lipid mediator formed from arachidonic acid. LTB<jats:sub>4<\/jats:sub> is among the most potent stimulants of polymorphonuclear leukocytes (PMNs) and, thus, participates in tissue injury by recruiting PMNs in a pathophysiologic scenario of periodontal diseases. The aim of the present study was to assess the relationship between clinical parameters and concentrations of LTB<jats:sub>4<\/jats:sub> within gingival crevicular fluid (GCF) from inflamed gingiva and periodontitis sites before and after the treatment of periodontitis.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Sixty subjects were divided into three groups with 20 subjects in each group: healthy (group 1), gingivitis (group 2), and chronic periodontitis (group 3). Groups were based on periodontal disease index (PDI), clinical attachment loss (CAL), and radiographic evidence of bone loss. Group 4 consisted of the subjects in group 3 at 6 to 8 weeks after treatment, i.e., scaling and root planing (SRP). GCF samples collected from each patient were quantified for LTB<jats:sub>4<\/jats:sub> using an enzymatic immunometric assay. In addition, the correlation between in situ LTB<jats:sub>4<\/jats:sub> levels and clinical parameters was analyzed in each group.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> The highest mean LTB<jats:sub>4<\/jats:sub> concentration in GCF was observed in group 3 (185.2 pg\/\u03bcl), and the lowest was observed in group 1 (39.6 pg\/\u03bcl). Its level in group 3 decreased to 79.35 pg\/\u03bcl after treatment (group 4). Further, GCF LTB<jats:sub>4<\/jats:sub> levels in all groups showed a statistically significant positive correlation with PDI and CAL (<jats:italic>P<\/jats:italic> &lt;0.005).<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> The substantial increase in GCF LTB<jats:sub>4<\/jats:sub> concentrations with the severity of periodontal disease and a concomitant decrease in its level following SRP in subjects with periodontitis suggest a possible role for LTB<jats:sub>4<\/jats:sub> in the progression of periodontal disease.<\/jats:p>","DOI":"10.1902\/jop.2007.070135","type":"journal-article","created":{"date-parts":[[2007,11,30]],"date-time":"2007-11-30T08:34:09Z","timestamp":1196411649000},"page":"2325-2330","source":"Crossref","is-referenced-by-count":18,"title":["Gingival Crevicular Fluid Levels of Leukotriene B<sub>4<\/sub> in Periodontal Health and Disease"],"prefix":"10.1002","volume":"78","author":[{"given":"A.R.","family":"Pradeep","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.G.","family":"Manjunath","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pradeep Patel","family":"Swati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chowdhry","family":"Shikha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pai B.","family":"Sujatha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,12]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1997.tb01394.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6254151"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2001.72.8.1025"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1991.tb01649.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2820055"},{"key":"e_1_2_8_7_1","first-page":"1","article-title":"Leukotriene B4 in inflammation","volume":"10","author":"Ford\u2010Hutchinson AW","year":"1990","journal-title":"Crit Rev Immunol"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910308200202"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0192-0561(92)90174-J"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1997.2.1.123"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3109\/00016356309011240"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1967.38.6.602"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-5712(96)00084-X"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1993.tb02090.x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0090-6980(88)90301-2"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.4.1453-1459.1993"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0757.2003.03103.x"},{"key":"e_1_2_8_18_1","first-page":"501","article-title":"Age\u2010dependent change in arachidonic acid metabolic capacity in rat alveolar macrophages","volume":"47","author":"Chakraborti T","year":"1999","journal-title":"Biochem Mol Biol Int"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000092941.77774.3C"},{"key":"e_1_2_8_20_1","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1016\/S0022-3565(24)29557-4","article-title":"In vitro and in vivo pharmacological characterization of BIIL 284, a novel and potent leukotriene B4 receptor antagonist","volume":"297","author":"Birke FW","year":"2001","journal-title":"J Pharmacol Exp Ther"}],"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2007.070135","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T09:09:47Z","timestamp":1760864987000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2007.070135"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,12]]},"references-count":19,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2007,12]]}},"alternative-id":["10.1902\/jop.2007.070135"],"URL":"https:\/\/doi.org\/10.1902\/jop.2007.070135","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"type":"print","value":"0022-3492"},{"type":"electronic","value":"1943-3670"}],"subject":[],"published":{"date-parts":[[2007,12]]}}}