{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T06:15:27Z","timestamp":1789193727379,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,1,1]],"date-time":"2008-01-01T00:00:00Z","timestamp":1199145600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> <jats:italic>Helicobacter pylori<\/jats:italic> has been associated with the development of peptic ulcers and gastric cancer. Although the oral cavity may be a source of transmission, it is unknown whether it acts as a permanent reservoir for this bacterium, particularly in the presence of periodontal disease. The purpose of this study was to evaluate the prevalence of <jats:italic>H. pylori<\/jats:italic> by polymerase chain reaction (PCR) in the subgingival biofilm and saliva of subjects with periodontitis.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Samples were obtained from 56 periodontally healthy subjects and 169 subjects with chronic periodontitis. DNA was extracted from the samples, and the detection of <jats:italic>H. pylori<\/jats:italic> was carried out by PCR using the JW22\/23 primers.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> In general, <jats:italic>H. pylori<\/jats:italic> was detected in 24% of all samples evaluated. A significantly higher prevalence of <jats:italic>H. pylori<\/jats:italic> was observed in subgingival biofilm samples (33.3%) compared to saliva samples (20%) (<jats:italic>P<\/jats:italic> &lt;0.05). <jats:italic>H. pylori<\/jats:italic> was detected significantly more often in the saliva and subgingival samples from subjects with periodontitis (23.5% and 50%, respectively) compared to samples from periodontally healthy subjects (7.3% and 11.4%, respectively; <jats:italic>P<\/jats:italic> &lt;0.05).<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> <jats:italic>H. pylori<\/jats:italic> was detected frequently in the oral microbiota of subjects with periodontitis, suggesting that periodontal pocketing and inflammation may favor the colonization by this species.<\/jats:p>","DOI":"10.1902\/jop.2008.070241","type":"journal-article","created":{"date-parts":[[2007,12,31]],"date-time":"2007-12-31T09:29:20Z","timestamp":1199093360000},"page":"97-103","source":"Crossref","is-referenced-by-count":60,"title":["Detection of <i>Helicobacter pylori<\/i> by Polymerase Chain Reaction in the Subgingival Biofilm and Saliva of Non\u2010Dyspeptic Periodontal Patients"],"prefix":"10.1002","volume":"79","author":[{"given":"Renata","family":"Souto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula 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