{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:49:40Z","timestamp":1791269380563,"version":"4.1.0"},"reference-count":51,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2008,3,1]],"date-time":"2008-03-01T00:00:00Z","timestamp":1204329600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100008364","name":"Tandl\u00e6geforeningen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008364","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2008,3]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Microarray analysis of local and peripheral cells in subjects with immune\u2010inflammatory diseases may identify candidate genes associated with these diseases. The present study identified differentially expressed genes in peripheral blood mononuclear cells (PBMCs) from subjects with untreated localized aggressive periodontitis (LAgP) or generalized aggressive periodontitis (GAgP). Differentially expressed genes were validated in groups of subjects with LAgP, GAgP, juvenile idiopathic arthritis (JIA), or rheumatoid arthritis (RA) and controls.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Candidate genes were identified by gene expression profiling of PBMCs using a microarray system in untreated gender\u2010matched subjects with LAgP (N = 2) or GAgP (N = 3) and controls (N = 2) younger than 35 years of age. The microarray results were validated by real\u2010time reverse transcription\u2010polymerase chain reaction (RT\u2010PCR) using PBMCs from 103 individuals, including groups of subjects with LAgP (N = 18), GAgP (N = 27), JIA (N = 10), or RA (N = 23) and controls (N = 25).<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Of 53 differentially expressed candidate genes identified in subjects with LAgP, 14 were involved in immune responses and inflammatory processes. Of these, the RT\u2010PCR validation confirmed that Toll\u2010like receptor 2 gene (<jats:italic>TLR2<\/jats:italic>) and <jats:italic>myomesin 2<\/jats:italic> gene had a significantly higher expression in subjects with LAgP than in controls. RT\u2010PCR also showed increased expression of <jats:italic>TLR2<\/jats:italic> in subjects with RA. Comparison of subjects with GAgP to controls using microarray analysis identified only three upregulated genes.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> Several genes upregulated in subjects with LAgP were related to immune responses including <jats:italic>TLR2<\/jats:italic> and <jats:italic>myomesin 2<\/jats:italic>.<\/jats:p>","DOI":"10.1902\/jop.2008.070309","type":"journal-article","created":{"date-parts":[[2008,3,3]],"date-time":"2008-03-03T09:21:35Z","timestamp":1204536095000},"page":"477-485","source":"Crossref","is-referenced-by-count":34,"title":["Blood Cell Gene Expression Profiling in Subjects With Aggressive Periodontitis and Chronic Arthritis"],"prefix":"10.1002","volume":"79","author":[{"given":"Lars 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