{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T08:34:51Z","timestamp":1791102891651,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2008,7,1]],"date-time":"2008-07-01T00:00:00Z","timestamp":1214870400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004921","name":"Shanghai Jiao Tong University","doi-asserted-by":"publisher","award":["BXJ0728"],"award-info":[{"award-number":["BXJ0728"]}],"id":[{"id":"10.13039\/501100004921","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2008,7]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Current epidemiologic data suggest that a localized infection (periodontitis) can disseminate into the distant tissues, and subgingival bacteria can migrate in the bloodstream, thereby contributing to independent systemic disease processes. To test this hypothesis, we investigated the effect of repeated systemic inoculations with <jats:italic>Porphyromonas gingivalis<\/jats:italic> (<jats:italic>Pg<\/jats:italic>) on intimal hyperplasia in iliac arteries in a rabbit model of balloon injury.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> One week after single balloon injury to the iliac artery, 30 male New Zealand rabbits were randomly assigned to intravenous inoculation with 100 \u03bcl live <jats:italic>Pg<\/jats:italic> (10<jats:sup>7<\/jats:sup> colony\u2010forming units; n = 15) or vehicle (n = 15) once weekly for 4, 8, or 12 consecutive weeks. Arteries were fixed by perfusion and removed for analysis of neointimal lesion formation. We measured intimal and medial lesion areas in iliac artery cross\u2010sections as well as the intimal\/medial ratio (I\/M). We also analyzed <jats:italic>Pg<\/jats:italic> 16S ribosomal DNA amplification with polymerase chain reaction, systemic proinflammatory mediators with enzyme\u2010linked immunosorbent assay, and immunolocalization of macrophages in the balloon\u2010injured arteries.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> At 12 weeks, iliac intimal hyperplasia was accelerated, and I\/M was significantly increased in <jats:italic>Pg<\/jats:italic>\u2010inoculated animals (I\/M 3.961 \u00b1 0.536 in the <jats:italic>Pg<\/jats:italic> group versus 3.585 \u00b1 0.353 in the control animals; <jats:italic>P<\/jats:italic> &lt;0.01). <jats:italic>Pg<\/jats:italic>\u2010inoculated animals also had significant increases in macrophage infiltration at 12 weeks, C\u2010reactive protein levels at all time points, and interleukin\u20106 levels at 12 weeks. Moreover, <jats:italic>Pg<\/jats:italic> ribosomal DNA was found in the injured arteries of <jats:italic>Pg<\/jats:italic>\u2010inoculated animals, but only after 12 weeks.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> Long\u2010term systemic challenge with <jats:italic>Pg<\/jats:italic>, an oral pathogen, may accelerate intimal hyperplasia in balloon\u2010injured iliac arteries.<\/jats:p>","DOI":"10.1902\/jop.2008.070425","type":"journal-article","created":{"date-parts":[[2008,7,2]],"date-time":"2008-07-02T08:54:28Z","timestamp":1214988868000},"page":"1192-1199","source":"Crossref","is-referenced-by-count":13,"title":["<i>Porphyromonas gingivalis<\/i> Infection Accelerates Intimal Thickening in Iliac Arteries in a Balloon\u2010Injured Rabbit 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