{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T01:11:02Z","timestamp":1773105062877,"version":"3.50.1"},"reference-count":46,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2008,10,1]],"date-time":"2008-10-01T00:00:00Z","timestamp":1222819200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2008,10]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> The goal of this study was to evaluate clinically and histologically the healing of advanced intrabony defects following regenerative periodontal surgery with an enamel matrix derivative (EMD) combined with a new biphasic calcium phosphate (BCP).<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Ten subjects, each of them displaying advanced combined 1\u2010 and 2\u2010wall intrabony defects around teeth scheduled for extraction because of advanced chronic periodontitis and further prosthodontic considerations, were included in the study. The defects were consecutively treated with a combination of EMD + BCP. A notch was placed at the most apical extent of the calculus present on the root surface or at the most apical part of the defect (if no calculus was present) to serve as a reference for the histologic evaluation. At 9 months after regenerative surgery, nine of 10 teeth were extracted with some of their surrounding soft and hard tissues and processed for histologic evaluation.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> There were no adverse effects related to EMD or the graft material used in any of the treated subjects. One tooth was not extracted because of the excellent clinical outcome. The clinical measurements at the nine biopsied teeth demonstrated a mean probing depth reduction of 3.3 \u00b1 1.4 mm and a mean clinical attachment level gain of 3.0 \u00b1 1.6 mm. The histologic findings indicated formation of cementum with inserting collagen fibers to a varying extent. A long junctional epithelium was observed in three of the nine biopsies. Mean new connective tissue attachment (i.e., new cementum with inserting collagen fibers) varied from 0.0 to 2.1 mm. The amount of newly formed bone was limited and varied from 0.0 to 0.7 mm. At 9 months, graft particles were still present and were mostly encapsulated in connective tissue, whereas formation of bone around the graft particles was observed only occasionally. Direct contact between the graft particles and the root surface (cementum or dentin) was not observed in any of the analyzed specimens.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> The combination of EMD with a BCP bone substitute did not interfere with the regenerative potential reported for EMD and may result in formation of new cementum with an associated periodontal ligament. However, the combination of EMD + BCP resulted in no to minimal new bone formation.<\/jats:p>","DOI":"10.1902\/jop.2008.080009","type":"journal-article","created":{"date-parts":[[2008,10,3]],"date-time":"2008-10-03T09:21:16Z","timestamp":1223025676000},"page":"1991-1999","source":"Crossref","is-referenced-by-count":68,"title":["Clinical and Histologic Evaluation of an Enamel Matrix Derivative Combined With a Biphasic Calcium Phosphate for the Treatment of Human Intrabony Periodontal 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